ZMS1133
Anti-MUC5AC Antibody, clone CLH2 ZooMAb® Mouse Monoclonal
recombinant, expressed in HEK 293 cells
Synonym(s):
Mucin-5AC;MUC-5AC;Gastric mucin;Major airway glycoprotein;Mucin-5 subtype AC tracheobronchial;Tracheobronchial mucin;TBM
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About This Item
UNSPSC Code:
12352203
biological source
mouse
Quality Level
recombinant
expressed in HEK 293 cells
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
CLH2, recombinant monoclonal
product line
ZooMAb® learn more
form
lyophilized
mol wt
calculated mol wt 585.57 kDa
purified by
using protein G
species reactivity
human, mouse
packaging
antibody small pack of 25 μL
greener alternative product characteristics
Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
enhanced validation
recombinant expression
Learn more about Antibody Enhanced Validation
technique(s)
flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
isotype
IgG1
epitope sequence
Internal
Protein ID accession no.
UniProt accession no.
greener alternative category
, Aligned
shipped in
ambient
storage temp.
2-8°C
target post-translational modification
unmodified
Gene Information
mouse ... MUC5AC(4586)
General description
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Specificity
Clone CLH2 is a ZooMAb® Mouse recombinant monoclonal antibody that specifically detects Mucin 5AC (MUC5AC).
Immunogen
A synthetic peptide of human MUC5AC tandem repeats.
Application
Quality Control Testing
Evaluated by Immunohistochemistry (Paraffin) in human stomach tissue sections.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution of this antibody detected MUC5AC in human stomach tissue sections.
Tested Applications
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected MUC5AC in A549 cells.
Flow Cytometry Analysis: 1 μg from a representative lot detected MUC5AC in one million A549 cells.
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected MUC5AC in lysates from mouse stomach tissue and A549 cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Evaluated by Immunohistochemistry (Paraffin) in human stomach tissue sections.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution of this antibody detected MUC5AC in human stomach tissue sections.
Tested Applications
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected MUC5AC in A549 cells.
Flow Cytometry Analysis: 1 μg from a representative lot detected MUC5AC in one million A549 cells.
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected MUC5AC in lysates from mouse stomach tissue and A549 cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Target description
Mucin-5AC (UniProt: P98088; also known as MUC5-AC, Gastric mucin, Major airway glycoprotein 1, Mucin-5 subtype AC tracheobronchial, Tracheobronchial mucin 1. TBM) is encoded by the MUC5AC (also known as MUC5) gene (Gene ID: 4586) in human. MUC5-AC is a gel-forming glycoprotein of gastric and respiratory tract epithelia that protects the mucosa from infection and chemical damage by binding to inhaled microorganisms and particles that are subsequently removed by mucociliary system. It is synthesized with a signal peptide (aa 1-27), which is subsequently cleaved off to produce the mature form (aa 28-5654). MUC5-AC undergoes C-, O- and N-glycosylation. O-glycosylation occurs on the second and last threonine of the threonine/serine-rich tandem repeats TTPSPVPTTSTTSA. C-mannosylation in the cysteine-rich subdomains may be required for proper folding of these regions and for export from the endoplasmic reticulum during biosynthesis. At its N- and C-termini are larger and more complex domains that are rich in cysteine and are involved in formation of the mucin polymers. These domains include sequences, including the D1, D2, D , and D3 domains at the N-terminus and the D4, C, and CK domains at the C-terminus. MUC5-AC is overexpressed in a number of carcinomas. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Pothuraju, R., et al. (2020). Mol. Cancer, 19: Article 37; Perez-Vilar, J., et al. (2004). Glycobiology. 14(4); 325-337).
Physical form
Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
Reconstitution
300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Storage and Stability
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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