T7902

Thermolysin from Geobacillus stearothermophilus

powder, BioReagent, 30-350 units/mg protein (E1%/280), suitable for cell culture

• Synonym(s): Protease from Geobacillus stearothermophilus, Thermophilic-bacterial protease
• CAS Number: 9073-78-3
• Enzyme Commission number: 3.4.24.27 (BRENDA, IUBMB)
• EC Number: 232-973-4
• MDL number: MFCD00165549
• UNSPSC Code: 12352204
• NACRES: NA.75

Properties

• biological source: Geobacillus stearothermophilus
• product line: BioReagent
• form: powder
• specific activity: 30-350 units/mg protein (E1%/280)
• mol wt: 34.6 kDa
• purified by: crystallization
• technique(s): cell culture | mammalian: suitable; single cell analysis: suitable
• storage temp.: −20°C

Description

Application

A thermostable (thermophilic) extracellular metalloendopeptidase containing four calcium ions. Cofactors are zinc and calcium. Hydrolyzes protein bonds on the N-terminal side of hydrophobic amino acid residues. The pH optimum is 8.0 and the optimal temperature for activity is 70 °C. Considerably stable from pH 5 to 9.5. Thermolysin has a low cleavage specificity, therefore, it produces a number of short fragments that are suitable for sequencing. Preferential cleavage: X-cleavage-Y-Z where X=any amino acid; Y=Leu, Phe, Ile, Val, Met, Ala and Z is any amino acid other than Pro. Cleavage N-terminal to Leu is preferred over cleavage of N-terminal to Phe which is preferred over the others. Often used to do limited proteolysis for peptide mapping and studies of protein structure and conformational changes.

Unit Definition

One unit will hydrolyze casein to produce color equivalent to 1.0 μmole (181 μg) of tyrosine per min at pH 7.5 at 37 °C (color by Folin-Ciocalteu reagent).

Physical form

lyophilized powder containing calcium and sodium acetate buffer salts

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Statements: H334
• Precautionary Statements: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves