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SAB4700636

Sigma-Aldrich

Monoclonal Anti-HLA Class I-PE antibody produced in mouse

clone MEM-147, purified immunoglobulin, buffered aqueous solution

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

conjugate

phycoerythrin (R-PE) conjugate

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

MEM-147, monoclonal

form

buffered aqueous solution

species reactivity

human

concentration

0.1 mg/mL

technique(s)

flow cytometry: suitable

isotype

IgG1

NCBI accession no.

NM_002116.6
NM_002117.4
NM_005514.6

UniProt accession no.

P04439

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

Gene Information

human ... HLA-A(3105) , HLA-B(3106) , HLA-C(3107)

General description

The antibody MEM-147 reacts with all human classical MHC Class I molecules in native cell-surface forms (e.g. it recognizes native HLA-A2 in cytofluorometry and immunoprecipitation but not in Western blotting). MHC Class I molecules (MHC Class Ia) are expressed on the surface of all human nucleated cell types.

The previously assigned protein identifier P01891 has been merged into P04439. Full details can be found on the UniProt database.

Immunogen

PHA-activated peripheral blood lymphocytes

Application

The reagent is designed for Flow Cytometry analysis. Suggested working dilution is 5 μg/mL of sample. Indicated dilution is recommended starting point for use of this product. Working concentrations should be determined by the investigator.

Features and Benefits

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Physical form

Solution in phosphate buffered saline containing 15 mM sodium azide and 0.2% high-grade protease free BSA as a stabilizing agent.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

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K Drbal et al.
Blood, 98(5), 1561-1566 (2001-08-25)
An unusual CD18 monoclonal antibody (mAb) MEM-148 binds, in contrast to standard CD18 mAbs, specifically to peripheral blood monocytes and neutrophils activated by various stimuli such as phorbol myristate acetate, opsonized zymosan, heat-aggregated immunoglobulin, and (after priming with lipopolysaccharide, tumor

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