SAB4200824

Anti-TFE3 antibody, Mouse monoclonal

clone TFE3-37, purified from hybridoma cell culture

• Synonym(s): Class E basic helix-loop-helix protein 33, Transcription factor E3, bHLHe33
• UNSPSC Code: 12352203

Properties

• biological source: mouse
• antibody form: purified from hybridoma cell culture
• clone: TFE3-37, monoclonal
• form: buffered aqueous solution
• mol wt: 60 kDa
• species reactivity: human, mouse, bovine, rat, canine
• packaging: antibody small pack of 25 μL
• concentration: ~1 mg/mL
• technique(s): immunoblotting: 1.5-3 μg/mL using human A549 cells extract
• isotype: IgG1
• UniProt accession no.: P19532
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... TFE3(7030)

Description

General description

Transcription factor E3 (TFE3), also known as Class E basic helix-loop-helix protein 33 (bHLHe33), is a transcriptional activator that mediates the enhancer-promoter interactions. TFE3 belongs to the MiT family of helix-loop-helix leucine zipper transcription factors, it is ubiquitously expressed and can directly associate with DNA as either homodimer or heterodimer which are formed with two other MiT family members, TFEB or TFEC.
TFE3 serve an important role in cell growth, cell proliferation, cellular adaptation to starvation and cellular response to ER stress. Under nutrient-rich conditions, TFE3 is located in the cytoplasm, and under starvation conditions or treatment with ER stressors, TFE3 rapidly translocates to the nuclear. In the nuclear localization, TFE3 mediates cellular adaptation to stress by simultaneously promoting lysosomal biogenesis, autophagy induction, as well as expression of critical mitochondrial and metabolic regulators. It been shown that TFE3 also participate in the transcriptional regulation of the innate immune response.
Pathogen infections promote TFE3 nuclear translocation, thus inducing In vivo expression of several cytokines and chemokines.

Application

Anti-TFE3 antibody specifically recognizes TFE3 from human, mouse, rat, bovine and canine origin. The antibody may be used in various immunochemical techniques including Immunoblotting (~60 kDa). Detection of the TFE3 band by Immunoblotting is specifically inhibited by the immunizing peptide.

Physical form

Supplied as a solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide as a preservative.

Other Notes

Unless otherwise stated in our catalog our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable