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SAB4200588

Sigma-Aldrich

Anti-CHD1 (C-terminal) antibody from rabbit

affinity isolated antibody

Synonym(s):

ATP-dependent helicase CHD1, chromodomain helicase DNA binding protein 1

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About This Item

UNSPSC Code:
12352203

biological source

rabbit

Quality Level

200

antibody form

affinity isolated antibody

antibody product type

primary antibodies

form

buffered aqueous solution

species reactivity

human, mouse

concentration

~1.0 mg/mL

technique(s)

immunoblotting: 2.5-5.0 μg/mL using whole extracts of mouse P19 cells.
immunofluorescence: 2.5-5.0 μg/mL using human HeLa cells.

UniProt accession no.

O14646

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... CHD1(1105)
mouse ... Chd1(12648)

General description

Chromodomain-helicase-DNA-binding protein 1 (CHD1) is an ATP-dependent chromatin-remodeling factor. The CHD family of proteins contains two domains : chromo (chromatin organization modifier) and SNF2-related helicase/adenosine triphosphatase (ATPase).

Immunogen

synthetic peptide corresponding to the C-terminal region of human CHD1 , conjugated to KLH.

Application

Anti-CHD1 (C-terminal) antibody from rabbit has been used in immunoblotting and immunofluorescence.

Biochem/physiol Actions

Chromodomain-helicase-DNA-binding protein 1 (CHD1) modifies chromatin structure affecting the interaction of transcriptional apparatus to its chromosomal DNA template. CHD1 is required for maintaining open chromatin and pluripotency in embryonic stem cells (ESCs).

Physical form

a solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Glenn Hauk et al.
Current opinion in structural biology, 21(6), 719-727 (2011-10-15)
This review focuses on recent structural insights into regulation and nucleic acid binding of Superfamily 2 (SF2)-type helicases as they relate to chromatin remodelers. We review structural features of the Chd1 chromatin remodeler regarding regulation of the ATPase motor, and
Lena Ho et al.
Nature, 463(7280), 474-484 (2010-01-30)
New methods for the genome-wide analysis of chromatin are providing insight into its roles in development and their underlying mechanisms. Current studies indicate that chromatin is dynamic, with its structure and its histone modifications undergoing global changes during transitions in
Concetta G A Marfella et al.
Mutation research, 618(1-2), 30-40 (2007-03-14)
Chromatin remodeling enzymes contribute to the dynamic changes that occur in chromatin structure during cellular processes such as transcription, recombination, repair, and replication. Members of the chromodomain helicase DNA-binding (Chd) family of enzymes belong to the SNF2 superfamily of ATP-dependent

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