PRS2003

Anti-ICAD antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

• Synonym(s): Anti-DFF45, Anti-DNA fragmentation factor, α-subunit
• MDL number: MFCD02096208
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: predicted mol wt 45 kDa
• species reactivity: mouse
• technique(s): immunofluorescence: suitable; immunohistochemistry: suitable; indirect ELISA: suitable; western blot: suitable
• UniProt accession no.: O54786
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: mouse ... Dffa(13347)

Description

General description

The gene DNA fragmentation factor subunit α (DFFA) is mapped to human chromosome 1p36.2. The encoded protein localizes to the chromatin fraction. It exist in association with DFF40, thereby forming a heterodimer. DFFA is popularly called as DFF45 (DNA fragmentation factor 45).

Immunogen

a peptide corresponding to amino acids 312-331 of mouse ICAD.

Biochem/physiol Actions

DNA fragmentation factor subunit α (DFFA) is a component of DNA fragmentation factor (DFF). It is mainly associated with apoptosis and genomic stability. Caspase-3-mediated cleavage of DFFA releases DFF40 (DNA fragmentation factor 40) which degrades chromosomal DNA. During menstruation, the apoptosis of endometrium cells is associated with DFFA. Its expression decreases after menopause. Silencing of DFFA increases doxorubicin effects in breast cancer cells.

Linkage

The action of this antibody can be blocked using blocking peptide SBP2003.

Physical form

Solution in phosphate buffered saline containing 0.02% sodium azide

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable