P3243
Phosphodiesterase I from Crotalus adamanteus venom
vial of ≥100 units, Purified
Synonym(s):
5′-Exonuclease, Oligonucleate 5′-nucleotidohydrolase
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About This Item
Recommended Products
biological source
Crotalus adamanteus venom
Quality Level
form
solid
quality
Purified
specific activity
≥20.0 unit/mg solid
packaging
vial of ≥100 units
storage temp.
−20°C
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Application
Phosphodiesterase (PDE) is any enzyme that is used to breaks phosphodiester bonds. It is a membrane-bound glycoprotein that is used to catalyze the hydrolysis of various nucleotide polyphosphates. Phosphodiesterase I is used in phosphodiesterase activation assays to hydrolyze AMP. Product P3243 is from Crotalus adamanteus venom and is purified. Product P3243 has been used to hydrolyze tRNA with wyosine derivatives into mononucleosides.
Biochem/physiol Actions
Phosphodiesterase I breaks phosphodiester bonds and catalyzes the hydrolysis of various nucleotide polyphosphates. Phosphodiesterase I is released from eucaryotic plasma membranes by phosphatidylinositol-specific phospholipase C.
Unit Definition
One Unit hydrolyzes one μmole of p-nitrophenyl thymidine-5-phosphate per minute at 25 °C, pH 8.9
Preparation Note
Purified via the method of Williams, et al. and further treated to inactivate contaminating 5′-nucleotidase activity.
Reconstitution
For testing purposes, PDE I is reconstituted in cold deionized water at 0.1 - 0,2 un/mL.
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1
Storage Class Code
13 - Non Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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Action of venom phosphodiesterase on deoxyribonucleic acid.
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Protocols
Enzymatic Assay of 5’-Nucleotidase
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