Skip to Content
Merck
All Photos(1)

Key Documents

View All Documentation

O4387

Sigma-Aldrich

Oligo(dT)23, Anchored

70 μM in H2O

Synonym(s):

Oligo(dT)23 Anchored Primer

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

UNSPSC Code:
41106305
NACRES:
NA.52

biological source

synthetic (organic)

Quality Level

200

form

solution

usage

0.1 mL sufficient for 100 RT-PCR reactions (as described in the Technical Bulletin for Product Codes HSRT100 and HSRT20)

concentration

70 μM in H2O

color

colorless

shipped in

wet ice

storage temp.

−20°C

Related Categories

General description

Oligo(dT)23, Anchored primers are used to prime mRNA with a poly(A) tail for cDNA synthesis. The primers have 23 thymidine residues and one G, C, or A residue (the anchor) at the 3′ end. This anchor ensures that the oligo(dT) primer binds at the very beginning of the message and there is not a long region of the unusable sequence. The optimal conditions for the concentration of enhanced AMV reverse transcriptase, template RNA, primers, and amplification parameters will depend on the system being utilized and should be determined empirically.

Application

Oligo(dT)23, Anchored has been used in the synthesis of cDNA for real-time quantitative polymerase chain reaction (PCR).
The Anchored Oligo(dT)23 Primers have 23 thymidine residues and one G, C, or A residue (the anchor) at the 3′ end. This anchor ensures the oligo(dT)23 primer binds at the beginning of the message such that there are no long regions of unusable sequence. Anchored oligo(dT)23 primers may provide an advantage over standard oligo(dT) primers when generating cDNA from poly(A)+ RNA.

Features and Benefits

  • In the process of preparing cDNA libraries, when there is incomplete or missing sequence information or when specific primers are not suitable, Oligo(dT)23, Anchored primers can be employed together with random nonamers (Product No. R 7647).
  • These primers can serve as a substitute for reverse transcription primers in tasks like first-strand cDNA synthesis, cDNA library construction, and various other applications.
  • Following transcription, the anchored oligo(dT)23 primers exhibit a reduced ability to initiate priming at higher temperatures (up to 65 °C), ensuring that they do not interfere with PCR.
  • Anchored oligo(dT)23 primers may provide an advantage over standard oligo(dT) primers when generating cDNA from poly(A)+ RNA.

Other Notes

For laboratory use only. Not for drug, household or other uses.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Leila Azimi et al.
Gene, 576(1 Pt 1), 166-170 (2015-10-13)
Carbapenemase production causes multi antibiotics resistant in Gram-negative bacteria. A simple rapid and accurate phenotypic test for detection of Gram-negative carbapenemase-producing bacteria is useful for the treatment of infections. The aim of this study was to track the negative results
Camilla Kwong et al.
PLoS genetics, 4(9), e1000178-e1000178 (2008-09-06)
Polycomb-group (PcG) and Trithorax-group proteins together form a maintenance machinery that is responsible for stable heritable states of gene activity. While the best-studied target genes are the Hox genes of the Antennapedia and Bithorax complexes, a large number of key
Lunda Gu et al.
PloS one, 11(1), e0146645-e0146645 (2016-01-12)
Epigenetic marks critically control gene expression and thus the cellular activity state. The functions of many epigenetic modifiers in the vascular system have not yet been studied. We screened for histone modifiers in endothelial cells and observed a fairly high
P Goelet et al.
Proceedings of the National Academy of Sciences of the United States of America, 79(19), 5818-5822 (1982-10-01)
Oligonucleotide primers have been used to generate a cDNA library covering the entire tobacco mosaic virus (TMV) RNA sequence. Analysis of these clones has enabled us to complete the viral RNA sequence and to study its variability within a viral
Andrea E Vasconez et al.
Acta physiologica (Oxford, England), 225(1), e13168-e13168 (2018-08-05)
The histone demethylase Jarid1b limits gene expression by removing the active methyl mark from histone3 lysine4 at gene promoter regions. A vascular function of Jarid1b is unknown, but a vasoprotective function to inflammatory and hypertrophic stimuli, like angiotensin II (AngII)
View All Related Papers

Articles

Reverse Transcription

One approach to the analysis of gene expression is to measure the concentration of mRNA of a gene. There are several challenges to such analyses, such as the differences in half life between different transcripts, the temporal patterns of transcription and the lack of correlation between mRNA and protein.

Protocols

The 3'/5' Assay for Analysis of RNA Integrity Protocol

The 3’/5’ integrity assay is a potential first step in the identification of RNA degradation. The assay is particularly useful when a large number of samples are to be analyzed or when the degradation is less than that detected by capillary systems but still sufficient to effect qPCR analyses.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service