MAK121

Neuraminidase Activity Assay Kit

sufficient for 100 colorimetric or fluorometric tests

• Synonym(s): Sialidase Activity Assay Kit
• UNSPSC Code: 12161503
• NACRES: NA.84

Properties

• usage: sufficient for 100 colorimetric or fluorometric tests
• application(s): pharmaceutical
• detection method: colorimetric; fluorometric
• shipped in: dry ice
• storage temp.: −20°C

Description

General description

Neuraminidase, also known as Sialidase, is an enzyme that hydrolyzes terminal sialic acid residues on poly-saccharide chains. It is predominantly expressed in microorganisms such as bacteria and viruses. Cleavage of sialic acid residues by neuraminidase is believed to play several roles in infection by influenza viruses. It is thought to assist in the penetration of mucosal linings, the invasion of target cells, the elution of progeny viruses from infected cells, and the prevention of self-aggregation. Thus, neuraminidase is an important target for influenza drug development and simple, direct, and automation-ready procedures for measuring neuraminidase activity find wide applications in research and drug discovery.

Application

• 5-Nitrobenzo[c][1, 2, 5]selenadiazole as therapeutic agents in the regulation of oxidative stress and inflammation induced by influenza A(H1N1)pdm09 in vitro and in vivo.: This research utilized the Neuraminidase Activity Assay Kit to explore therapeutic agents that regulate oxidative stress and inflammation, showcasing its crucial role in antiviral research and potential pharmacological applications (Guo et al., 2023).


• Potency of bacterial sialidase Clostridium perfringens as antiviral of Newcastle disease infections using embryonated chicken egg in ovo model.: Highlights the kit′s use to measure and observe sialidase enzyme activity to assess the antiviral potential of bacterial enzymes against poultry diseases, contributing to the development of novel biocontrol methods (Kurnia et al., 2022).


• Molecular Docking Studies and Biological Evaluation of Berberine-Benzothiazole Derivatives as an Anti-Influenza Agent via Blocking of Neuraminidase.: Utilizes neuraminidase assay kit to evaluate the efficacy of synthesized compound′s neuraminidase activity, offering a path towards the design of new antiviral drugs with enhanced specificity and potency (Kumar et al., 2021).

Features and Benefits

Compatible with high-throughput handling systems.

Suitability

Suitable for the detection of neuraminidase activity in biological samples and for the screening of neuraminidase inhibitors.

Principle

In this assay, neuraminidase activity is determined by an enzymatic assay in which sialic acid released by neuraminidase results in a colorimetric (570 nm)/fluorometric (λex = 530/λem = 585 nm) product directly proportional to neuraminidase activity in the sample. The linear detection range at 37 °C is 0.1-10 units/L for colorimetric assays and 0.01-2 units/L for fluorometric assays.

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Statements: H317,H334
• Precautionary Statements: P261 - P272 - P280 - P284 - P302 + P352 - P333 + P313
• Hazard Classifications: Resp. Sens. 1 - Skin Sens. 1
• Storage Class Code: 10 - Combustible liquids