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H9286

Anti-acetyl-Histone H3 (Ac-Lys⁹) antibody produced in rabbit

Name: Anti-acetyl-Histone H3 (Ac-Lys<SUP>9</SUP>) antibody produced in rabbit
Brand: SIGMA

2-2.5 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym(s):

Anti-H3K9ac

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About This Item

MDL number:

MFCD02685921

UNSPSC Code:

12352203

NACRES:

NA.41

biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~17 kDa

species reactivity

bovine, human, Caenorhabditis elegans, frog, mouse, rat, Drosophila, chicken

concentration

2-2.5 mg/mL

technique(s)

indirect immunofluorescence: 1:1,000 using NIH3T3 mouse fibroblast cell line treated with sodium butyrate
microarray: suitable
western blot: 1:1,000 using whole cell extract of the mouse fibroblast NIH3T3 cell line treated with sodium butyrate

UniProt accession no.

P68431

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

acetylation (Lys9)

Gene Information

human ... H3F3A(3020) , H3F3B(3021) , HIST1H3A(8350) , HIST1H3B(8358) , HIST1H3C(8352) , HIST1H3D(8351) , HIST1H3E(8353) , HIST1H3F(8968) , HIST1H3G(8355) , HIST1H3H(8357) , HIST1H3I(8354) , HIST1H3J(8356) , HIST2H3A(333932) , HIST2H3C(126961) , HIST3H3(8290)
mouse ... H3f3a(15078) , H3f3b(15081) , Hist1h3a(360198) , Hist1h3b(319150) , Hist1h3c(319148) , Hist1h3d(319149) , Hist1h3e(319151) , Hist1h3f(260423) , Hist1h3g(97908) , Hist1h3h(319152) , Hist1h3i(319153) , Hist2h3b(319154) , Hist2h3c1(15077) , Hist2h3c2(97114)
rat ... H3f3b(117056)

General description

Anti-acetyl-Histone H3 (Ac-Lys9) antibody produced in rabbit is suitable for:

indirect immunofluorescence at a dilution of 1:1,000 using NIH3T3 mouse fibroblast cell line treated with sodium butyrate
microarray
western blot at a dilution of 1:1,000 using whole cell extract of the mouse fibroblast NIH3T3 cell line treated with sodium butyrate

Immunogen

synthetic acetylated [Ac-Lys⁹] histone H3 peptide (amino acids 7-20) corresponding to the N-terminus of human histone H3. The sequence is identical in many species including mouse, rat, bovine, chicken, frog, Drosophila, and C. elegans, and is highly conserved (single amino acid substitution) in Tetrahymena histone H3.

Application

Anti-acetyl-Histone H3 (Ac-Lys9) antibody produced in rabbit has been used in:

indirect immunofluorescence
immunoblotting
immunohistochemistry

Biochem/physiol Actions

Histone proteins H3, H4, H2A, and H2B function as building blocks to package eukaryotic DNA into repeating nucleosome units that are folded in higher order chromatin fibers.

In most species, histone H3 is primarily acetylated at lysine 9, 14, 18, and 23. Acetylation at lysine 9 significantly influences histone deposition and chromatin assembly in some organisms. Acetylation of specific lysine residues in H3 is also associated with processes apart from transcription.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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