GE17-1179-01
Resource™ Q
Cytiva 17-1179-01, pack of 6 mL
Synonym(s):
Resource Q Column
About This Item
Recommended Products
ligand
quaternary ammonium
description
Ion Exchanger Type (value)
packaging
pack of 6 mL
manufacturer/tradename
Cytiva 17-1179-01
parameter
87 psi
bed size
16 mm × 30 mm
bed volume
6 mL
matrix
polystyrene/divinylbenzene
avg. part. size
15 μm
particle size
15-15 μm
cleaning
1-14
working range
2-12
suitability
suitable for bioprocess medium
Related Categories
General description
The small (15 μm) monodisperse bead give high-resolution purification at high flow rates. In addition, hydrophilization of the beads minimizes nonspecific adsorption and allows high recovery of purified sample. The material of the column body is PEEK (polyetheretherketone). SOURCE™ 30Q and 30S media with a 30 μm bead size are designed for intermediate purification and large-scale polishing and allow higher flow rates.
Application
This application note describes a convenient and simple protocol for the purification of synthetic phosporothioate oligonucleotides, using ion exchange chromatography on an automated AKTAexplorer 100 system. These data show that AKTAexplorer 100, controlled by UNICORN, is a convenient way of obtaining reliable and reproducible results, which can also be scaled-up.
Features and Benefits
- SOURCE™ 15Q and 15S media are highly suitable for fast, high-resolution purifications and easy scale-up.
- Rigid, monodispersed, spherical particles with controlled pore-size distribution offer excellent flow characteristics, sample loading of up to 25 mg of protein/mL, and improved stability in organic solvents and pH extremes
- RESOURCE™ prepacked columns for fast separation. Separation times using 1 mL RESOURCE™ columns are less than 3 min at 9.6 mL/min and about 20 min using a peristaltic pump at 1 mL/min.
- Improved capacity compared to MonoBeads but with a slightly lower resolution.
Storage and Stability
Analysis Note
Legal Information
Signal Word
Warning
Hazard Statements
Precautionary Statements
Storage Class Code
3 - Flammable liquids
Certificates of Analysis (COA)
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Protocols
This page covers using SOURCE™ medias for the purification of proteins, peptides, or oligonucleotides.
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This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.
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