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GAHK20

Supelco

Glucose (HK) Assay Kit

sufficient for 20 assays

Synonym(s):

Glucose Quantification Kit

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About This Item

UNSPSC Code:
12164500
NACRES:
NA.84

usage

sufficient for 20 assays

Quality Level

100

analyte chemical class(es)

sugars (glucose)

technique(s)

photometry: suitable

application(s)

food and beverages
general analytical

storage temp.

2-8°C

Application

This kit is for the quantitative, enzymatic determination of glucose in food and other material. Glucose is phosphorylated by adenosine triphosphate (ATP) in the reaction catalyzed by hexokinase. Glucose-6-phosphate (G6P) is then oxidized to 6-phospho-gluconate in the presence of oxidized nicotinamide adenine dinucleotide (NAD) in a reaction catalyzed by glucose-6-phosphate dehydrogenase (G6PDH). During this oxidation, an equimolar amount of NAD is reduced to NADH. The consequent increase in absorbance at 340 nm is directly proportional to glucose concentration.

Kit Components Only

Product No.
Description
  • glucose (HK) assay reagent 20 mL
  • glucose standard .5 mL

Storage Class Code

10 - Combustible liquids

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Rebecca A Haeusler et al.
Nature communications, 5, 5190-5190 (2014-10-14)
Insulin integrates hepatic glucose and lipid metabolism, directing nutrients to storage as glycogen and triglyceride. In type 2 diabetes, levels of the former are low and the latter are exaggerated, posing a pathophysiologic and therapeutic conundrum. A branching model of
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Enzymatic methods for food analysis are highly specific and offer considerable time and cost savings over other methods, especially from the sample preparation standpoint. We offer a wide variety of convenient kits and reagents for rapid and reliable enzymatic food analysis.

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