G6278
Glycerokinase from Escherichia coli
300-600 units/mL, ammonium sulfate suspension
Synonym(s):
ATP:glycerol 3-phosphotransferase, Glycerol Kinase
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About This Item
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biological source
Escherichia coli
form
ammonium sulfate suspension
concentration
300-600 units/mL
storage temp.
2-8°C
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General description
Research Area: Cell Signaling
Glycerol kinase (GK) is a member of the FGGY carbohydrate kinase family (ATP: glycerol 3-phosphotransferase), which is responsible for transferring a phosphate group from ATP to glycerol.
Glycerol kinase (GK) is a member of the FGGY carbohydrate kinase family (ATP: glycerol 3-phosphotransferase), which is responsible for transferring a phosphate group from ATP to glycerol.
Application
Glycerol kinase (glpK) was used to study the effects of pain controlling neuropeptides on human fat cell lipolysis.
Glycerol kinase has been used along with adenosine triphosphate (ATP) for the conversion of glycerol to glycerol-3 phosphate (glycerol-3P) in white adipose tissue (WAT) adipocyte samples by label distribution method. It has also been used along with adenosine triphosphate (ATP) in the derivatization of glycerol to sn-glycerol-3 phosphate (glycerol-3P).
Biochem/physiol Actions
Glycerol kinase (GK), the primary enzyme involved in regulating glycerol uptake and its metabolism, was initially identified in E. coli. This enzyme acts as a catalyst in the Mg2+-ATP-dependent phosphorylation of glycerol to produce G3P, a crucial intermediate in various metabolic pathways, including the synthesis of glycerolipids and triglycerides, as well as glycogenesis, glycolysis, and gluconeogenesis.
GK participates in the glycerol 3-phosphate shuttle and, in conjunction with glycerol 3-phosphate dehydrogenase, converts glycerol into dihydroxyacetone phosphate. It plays a pivotal role in plasma glycerol withdrawal, glycerol utilization by different tissues, and in maintaining carbohydrate homeostasis. GK is positioned at the nexus of lipid and carbohydrate metabolism and may play a potential role in type 2 diabetes mellitus (T2DM).
GK participates in the glycerol 3-phosphate shuttle and, in conjunction with glycerol 3-phosphate dehydrogenase, converts glycerol into dihydroxyacetone phosphate. It plays a pivotal role in plasma glycerol withdrawal, glycerol utilization by different tissues, and in maintaining carbohydrate homeostasis. GK is positioned at the nexus of lipid and carbohydrate metabolism and may play a potential role in type 2 diabetes mellitus (T2DM).
Glycerol kinase catalyzes tge MgATP-dependent phosphorylation of glycerol to produce sn-glycerol-3-phosphate and is the rate limiting enzyme in the utilization of glycerol. It is also subject to feedback regulation by fructose-1,6-bisphosphate.
Unit Definition
One unit will convert 1.0 μmole of glycerol and ATP to L-α-glycerophosphate and ADP per min at pH 9.8 at 25 °C in a coupled system with PK/LDH.
Physical form
Suspension in 3.1 M (NH4)2SO4 pH 7.3, with 1% BSA and 2% trehalose
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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Articles
Instructions for working with enzymes supplied as ammonium sulfate suspensions
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