F3377
N-Formyl-L-methionine
≥90% (TLC)
Synonym(s):
fMet
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About This Item
Empirical Formula (Hill Notation):
C6H11NO3S
CAS Number:
Molecular Weight:
177.22
MDL number:
UNSPSC Code:
12352209
PubChem Substance ID:
NACRES:
NA.26
Recommended Products
product name
N-Formyl-L-methionine, ≥90% (TLC)
Quality Level
Assay
≥90% (TLC)
form
powder
color
white
storage temp.
−20°C
SMILES string
CSCC[C@H](NC=O)C(O)=O
InChI
1S/C6H11NO3S/c1-11-3-2-5(6(9)10)7-4-8/h4-5H,2-3H2,1H3,(H,7,8)(H,9,10)/t5-/m0/s1
InChI key
PYUSHNKNPOHWEZ-YFKPBYRVSA-N
Application
N-Formyl-L-methionine (fMet) is used to identify, differentiate and characterize amino acid N-deformylase(s), N-carbamoylase(s) and N-aminoacylase(s).
Packaging
Bottomless glass bottle. Contents are inside inserted fused cone.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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Dongli Pan et al.
Molecular cell, 25(4), 519-529 (2007-02-24)
Translocation requires large-scale movements of ribosome-bound tRNAs. Using tRNAs that are proflavin labeled and single-turnover rapid kinetics assays, we identify one or possibly two kinetically competent intermediates in translocation. EF-G.GTP binding to the pretranslocation (PRE) complex and GTP hydrolysis are
K Szkaradkiewicz et al.
European journal of biochemistry, 267(13), 4290-4299 (2000-06-24)
Two polypeptides resistant against proteolytic digestion were identified in Thermus thermophilus translation initiation factor 2 (IF2): the central part of the protein (domains II/III), and the C-terminal domain (domain IV). The interaction of intact IF2 and the isolated proteolytic fragments
Yuhei Chadani et al.
Molecular cell, 68(3), 528-539 (2017-11-04)
Nascent polypeptides can modulate the polypeptide elongation speed on the ribosome. Here, we show that nascent chains can even destabilize the translating Escherichia coli ribosome from within. This phenomenon, termed intrinsic ribosome destabilization (IRD), occurs in response to a special
T Chun et al.
The Journal of experimental medicine, 193(10), 1213-1220 (2001-05-23)
Major histocompatibility complex (MHC) class I-restricted CD8(+) T cells play a critical role in the protective immunity against Mycobacterium tuberculosis (Mtb). However, only a few Mtb peptides recognized by MHC class Ia-restricted CD8(+) T cells have been identified. Information on
J Tomsic et al.
The EMBO journal, 19(9), 2127-2136 (2000-05-03)
Binding of the 50S ribosomal subunit to the 30S initiation complex and the subsequent transition from the initiation to the elongation phase up to the synthesis of the first peptide bond represent crucial steps in the translation pathway. The reactions
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