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D7679

Sigma-Aldrich

3,3′-Diaminobenzidine (DAB) Liquid Substrate Dropper System

liquid

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About This Item

CAS Number:
7411-49-6
EC Number:
231-018-9
MDL number:
MFCD00012969
UNSPSC Code:
12352204
PubChem Substance ID:
24894154
NACRES:
NA.75

form

liquid

technique(s)

immunoblotting: suitable

solubility

water: soluble

shipped in

wet ice

storage temp.

2-8°C

SMILES string

Cl.Cl.Cl.Cl.Nc1ccc(cc1N)-c2ccc(N)c(N)c2

InChI

1S/C12H14N4.4ClH/c13-9-3-1-7(5-11(9)15)8-2-4-10(14)12(16)6-8;;;;/h1-6H,13-16H2;4*1H

InChI key

KJDSORYAHBAGPP-UHFFFAOYSA-N

General description

DAB (3,3′-Diaminobenzidine Tetrahydrochloride) Liquid Substrate Dropper System has been developed for use in immunohistology and immunoblotting procedures as a precipitating substrate for the detection of peroxidase activity. DAB is the immunohistology substrate of choice because it produces an intense brown stain that is easily observed. The end product is resistant to alcohol, therefore, a variety of counterstains and mounting media can be used with the DAB Liquid Substrate Dropper System. DAB Liquid Substrate Dropper System provides all of the chromogen and buffer/peroxide solutions needed to produce a fast and convenient DAB substrate solution.

Application

3,3′-Diaminobenzidine (DAB) Liquid Substrate Dropper System has been used as a developer in immunohistogical examination.

Caution

Stable at least one year at 2-8 °C.

Quantity

Each DAB liquid substrate dropper system will provide ten individual application dropper bottles of 10 mL of a fast and convenient DAB substrate solution.

Kit Components Only

Product No.
Description
  • 10× DAB Liquid Chromogen 10 mL
  • premeasured, ready-to-use buffer/peroxide solution for individual application 10 x 9

Pictograms

Health hazardExclamation mark
GHS08,GHS07

Signal Word

Danger

Hazard Statements

H302,H319,H341,H350

Hazard Classifications

Acute Tox. 4 Oral - Carc. 1B - Eye Irrit. 2 - Muta. 2

Storage Class Code

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 3

Certificates of Analysis (COA)

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N M Chu et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 37(2), 257-263 (1989-02-01)
Immunoblotting techniques are widely used for detection of antigen immobilized on nitrocellulose membranes. There are many immunolabeling methods and staining methods available to disclose the presence of antigen in such techniques. Five common staining methods each for alkaline phosphatase and
CD8+ T cells expressing IL-10 are associated with a favourable prognosis in lung cancer
Miotto D, et al.
Lung Cancer, 69(3), 355-360 (2010)
The duality of beta-catenin function: a requirement in lens morphogenesis and signaling suppression of lens fate in periocular ectoderm
Smith AN, et al.
Developmental Biology, 285(2), 477-489 (2005)
Carola W N Damen et al.
Analytical biochemistry, 393(1), 73-79 (2009-06-16)
For the bioanalysis of therapeutic monoclonal antibodies in biological matrices, immunoassays--especially enzyme-linked immunosorbent assays (ELISAs)--are the most widely used techniques. Although ELISAs are very sensitive, the obtained sensitivity is not always sufficient. In this study, we have investigated the possibilities
S M Hsu et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 30(10), 1079-1082 (1982-10-01)
Three metallic ions, NiCl2, CoCl2, and CuSO4, were found to modify the color of the normally brown diaminobenzidine (DAB) reaction. The colors ranged from purplish blue (NiCl2), dark blue/bluish black (CoCl2), to greyish blue (CuSO4). We have found that the
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