D5751
Blue Dextran
Void volume marker for gel filtration columns.
Synonym(s):
Dextran blue from Leuconostoc mesenteroides
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About This Item
biological source
non-animal source
Quality Level
form
powder
quality
200
mol wt
2,000,000
technique(s)
affinity chromatography: suitable
suitability
suitable for GFC marker
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General description
Blue Dextran is a soluble derivative of dextran. Its structure includes triazinyl dye Cibacron blue F3GA covalently bound to high molecular weight (2000 kDa) dextran. Blue Dextran is generally used as a marker to determine void volume in gel filtration chromatography columns.
Application
Blue Dextran has been used for calibration of size exclusion chromatography (SEC) column. It has also been used in Dextran blue permeability assays to determine the integrity of co-cultures containing epithelial cells. Blue dextran may be used in affinity chromatography, gel filtration chromatography, protein chromatography, and molecular weight markers. Blue dextran is also suitable to study Alzheimer′s disease.
Preparation Note
Prepared from dextran with an average mol. wt. of 2,000,000.
Analysis Note
degree of substitution: 0.10 to 0.12 mmol Reactive Blue 2 per gram of dextran
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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