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CLS3312

Corning® CellBIND® Surface CellSTACK® cell culture chambers

CellBIND® surface, polystyrene, sterile, 10 Chamber with Vent Caps, graduations, orange high-density polyethylene cap, case of 2

Synonym(s):

cell culture flasks, scale up cell culture flasks, tissue culture flasks

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About This Item

UNSPSC Code:
41122104
NACRES:
NB.13

material

orange high-density polyethylene cap
polystyrene
rectangular bottom flask
short neck

sterility

sterile

feature

cap vented
graduations

packaging

pack of 1
case of 2

manufacturer/tradename

Corning 3312

surface area

6360 cm2

working volume

1250-1900 mL

suitability

suitable for (scale-up cell culture)

binding type

CellBIND® surface

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General description

The Corning CellBIND surface is produced by a microwave process which makes a more hydrophilic surface giving more consistent, even cell attachment, increased cell growth and yields. CellBIND enhances cell attachment and growth under difficult conditions, including reduced serum or serum-free medium.

Both enzymatic and non-enzymatic dissociating solutions have been used to remove and harvest cells from CellBIND surfaces, scraping is not recommended due to the increased strength of cell attachment.
CellSTACK chambers are ideal for cell culture scale-up, providing cell yields in the 107 - 109 range and are useful alternatives to multiple roller bottles or spinner flasks.

  • Ideal for cell culture scale-up
  • useful alternatives to multiple roller bottles or spinner flasks
  • High mechanical strength and structural integrity
  • 100% leak tested
  • Certified nonpyrogenic and sterilized by gamma irradiation, with low particulate contamination from modern manufacturing methods
  • Easy to use larger openings with threaded closures and vented caps
  • Filling ports allow direct access to chamber bottom, with more options for sterile transfer of media and cultures

Features and Benefits

  • Has 6,360cm2 cell growth area
  • Two 26mm diameter filling ports allow direct access to chamber bottom providing greater flexibility for sterile filling and emptying by pouring, pipetting or via tubing in a fully closed system
  • Standard 3mm threaded caps have 0.2μm pore nonwettable membranes sealed directly to the caps to allow gas exchange while minimizing the risk of contamination
  • Optional 33mm threaded caps are available with integrally sealed USP Class VI certified C-FLEX tubing to allow direct sterile transfer of media and cells
  • USP Class VI polystyrene provides excellent optical clarity and mechanical strength
  • Sterilized by gamma radiation and certifiied nonpyrogenic
  • 100% leak tested prior to shipping
  • Treated with a patented CellBIND surface treatment for enhanced cell attachment.

Legal Information

CellBIND is a registered trademark of Corning, Inc.
CellSTACK is a registered trademark of Corning, Inc.
Corning is a registered trademark of Corning, Inc.

Certificates of Analysis (COA)

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Articles

Discover how the Corning CELLSTACK-5 Chamber is used to simply and enhance the mass production of recombinant proteins.

Protocols

This technical article covers the protocol for how to properly harvest cells from Corning® CellSTACK® culture chambers.

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