DLD-1

90102540, human colon, Epithelial

• UNSPSC Code: 41106514

Properties

• product name: DLD-1, 90102540
• biological source: human colon
• growth mode: Adherent
• karyotype: 2n = 46, pseudodiploid
• morphology: Epithelial
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Human colon adenocarcinoma

Cell Line Description

Derived from human colorectal adenocarcinoma. The cells have been used in the study of polar solvents on cell characteristics.The four cell lines: DLD-1 (90102540), HCT-15 (91030712), HCT-8 (90032006) and HRT-18 (86040306) have been shown to have a common genetic origin.

Application

DLD-1 cell line has been used to express shRNA oligos directed against coronin 2A (CRN5) and to study the role of CRN5 in tumor cell migration. It has also been used to determine the efficiency of antibody-coupled short interfering RNA (siRNA) for in vivo mRNA knockdown.

DNA Profile

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 8,11
D16S539: 12,13
D5S818: 13
D7S820: 10,12
THO1: 7,9.3
TPOX: 8,11
vWA: 18,19

Culture Medium

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-3 x 10,000 cells/cm² using 0.25% trypsin, 0.02% EDTA.

Other Notes

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