Neuro 2a Cell Line from mouse
89121404, mouse nerve, Neuronal/amoeboid-like
Synonym(s):
N2a Cells, Nb2a Cells, Neuro2a Cells
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About This Item
UNSPSC Code:
41106514
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product name
Neuro 2a Cell Line from mouse, 89121404
biological source
mouse nerve
description
Mouse Albino neuroblastoma
growth mode
Adherent
karyotype
2n = 40
morphology
Neuronal/amoeboid-like
products
Microtubular protein
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Mouse Albino neuroblastoma
Cell Line Description
Derived from a spontaneous tumour in an albino strain A mouse. Cells produce microtubular protein which is believed to play a role in the contractile system giving axoplasmic flow in nerve cells.
Application
Neuro 2a cell line has been used as a model to study the potential toxicological effects of copper oxide nanoparticles accumulation in the brain. It has also been used to determine the sensitivity of the cell line to saxitoxin.
Tumourigenicity studies, microtubular protein synthesis, vinblastine precipitation, GTP binding kinetics
Culture Medium
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:3 to 1:8 i.e. seeding at 4x10,000 cells cm2; 5% CO2; 37°C. After resuscitation cells can take up to 6 days before a monolayer forms. These cells only adhere to the culture flask very lightly, care should be taken when subculturing. There is no need to wash the cells with PBS or trypsin, merely pour off half of the spent medium carefully, give the flask a gentle knock and resuspend the cells in fresh medium using a pipette to gently disperse any clumps of cells to finally reseed at 1:2. On arrival, growing cultures should be spun out and re-seeded in fresh media.
Other Notes
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Certificates of Analysis (COA)
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