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Key Documents

DBS-FRhL-2

89101303, from rhesus monkey lung, Convention on the International Trade in Endangered Species of Wild Fauna and Flora (CITES)

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About This Item

UNSPSC Code:
41106514

biological source

rhesus monkey lung

growth mode

Adherent

karyotype

2n = 42, diploid no structural abnormalities observed

morphology

Fibroblast

receptors

Not specified

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Cell Line Origin

Monkey Rhesus lung

Cell Line Description

A diploid cell line derived from normal male Rhesus monkey fetus. Susceptible to poliovirus types 1-3, Coxsackie A9, parainfluenza 3, rubella and is non-tumourigenic in animals.

Culture Medium

EMEM (EBSS) (M2279) + 2 mM L-Glutamine (G7513) + 1% NEAA (M7145) + 10% FBS / FCS (F2442).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:2 to 1:3, i.e., seeding at 3-5x10,000 cells/cm2 using 0.25% trypsin; 5% CO2; 37 °C. Finite lifespan. Cell line has been shown to undergo 51 passages or 74 population doublings.

Other Notes

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Legal Information

This material is cited in a US and/or other Patent and may not be used to infringe parent claims. US Patent No. 4,040,905.

Certificates of Analysis (COA)

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