biological source
Mouse x Rat Hybridoma hybrid
growth mode
Adherent
karyotype
Not specified
morphology
Glial
products
IR-substance P-like material
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Mouse neuroblastoma x Rat glioma hybrid
Cell Line Description
A somatic hybrid derived by the hybridisation of the clonal mouse neuroblastoma N115TG-2 and the clonal rat glioma C6BV-1. The cells produce significant amounts of IR-substance P-like material.
Application
Hybridisation and fusion partner studies
Culture Medium
DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Other Notes
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Certificates of Analysis (COA)
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