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product name
CPAE, 86111401
biological source
bovine cardiovascular
growth mode
Adherent
karyotype
2n = 60, diploid
morphology
Endothelial
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Bovine pulmonary artery endothelium
Cell Line Description
Established from the main stem pulmonary artery of a young, female cow (Bos taurus). The cells have angiotensin-converting enzyme activity. BVDV positive.
Application
Enzymatic studies
Culture Medium
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 20% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Other Notes
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Certificates of Analysis (COA)
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