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Key Documents

AFRC MAC 77

Reactivity: Anti-Nitrate Reductase, 85102262

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About This Item

UNSPSC Code:
41106514

biological source

Rat x Rat Hybridoma

ImmunoDon

F344 rat spleen

Myeloma

Y3Ag1.2.3

Reactivity

Anti-Nitrate Reductase

technique(s)

cell culture | mammalian: suitable

isotype

IgG2b

shipped in

dry ice

storage temp.

−196°C

Related Categories

Cell Line Origin

Rat Y3Ag1.2.3 x Rat F344 spleen

Cell Line Description

The antibody specific for NADH nitrate reductase and inhibits the reduction of nitrate to nitrite using NADH as electron donor. It also inhibits the reduction of nitrate using flavin nucleotides (FMNH2 or FADH2) and methyl viologen. The antibody is directed against NADH-NR of mono and dicotyledons.

Immunogen

Nitrate reductase purified from the leaves of 6 to 8 week old spinach plants - Spinacea oleracea l

Culture Medium

DMEM + 2mM glutamine + 10% Foetal Bovine Serum (FBS)

Subculture Routine

Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. These cells require mouse macrophage feeder layers upon resuscitation

Other Notes

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