LIM2550

12070504, human colon, epithelial. Mixed adherent and suspension with floating aggregates.

• UNSPSC Code: 41106514

Properties

• product name: LIM2550, 12070504
• biological source: human colon
• description: Human columnar cell adenocarcinoma of the colon
• growth mode: Adherent
• morphology: Epithelial; cells grow as floating and loose aggregates with some adherent cells.
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Description

LIM2550 is derived from an adenocarcinoma of the colon. Cell Bank Australia reference number: CBA0169.

Culture Medium

RPMI1640 (with 2mM L-Glutamine + 25mM HEPES) + 10% FCS, Insulin 0.6μg/ml, Hydrocortisone 1μg/ml, 1-Thioglycerol 10 μM.

Subculture Routine

Optimal split ratio 1:4-1:8 (seeding density 1-2 x 10⁴ cells/cm²). Cells incubated at 37°C with 5% CO₂. Harvest the cells using 0.05% Trypsin/EDTA at 37°C for 5 min. LIM2550 requires the growth medium to be changed 3 times each week. Feed and passage the cells as for semi-adherent culture (collect medium with floating cells and then trypsinise the adherent population, pool both together before reseeding).

Other Notes

Cultures from PHE Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.