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product name
HCA-7 Colony 6, 09071504
biological source
human colon
growth mode
Adherent
karyotype
Near diploid
morphology
Epithelial
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Human colorectal adenocarcinoma
Cell Line Description
Subpopulations isolated from the HCA-7 cell line.
DNA Profile
STR-PCR Data:
Amelogenin: X
CSF1PO: 11,14
D13S317: 9
D16S539: 10,11
D5S818: 8,12
D7S820: 11,12
THO1: 6,7
TPOX: 8,10
vWA: 14,16
Amelogenin: X
CSF1PO: 11,14
D13S317: 9
D16S539: 10,11
D5S818: 8,12
D7S820: 11,12
THO1: 6,7
TPOX: 8,10
vWA: 14,16
Culture Medium
DMEM (High glucose Sigma D6546) + 2 mM Glutamine + 10% Fetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:5 to 1:10 weekly using 0.05% trypsin/EDTA; 7.5% CO2; 37 °C. Suggested seeding density 2-4x10,000 cells/cm2.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Disclaimer
This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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