C7988
Anti-Cre antibody, Mouse monoclonal
clone 7-23, purified from hybridoma cell culture
Synonym(s):
Mouse Anti-Recombinase cre
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About This Item
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biological source
mouse
conjugate
unconjugated
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
7-23, monoclonal
form
buffered aqueous solution
mol wt
antigen ~38 kDa
concentration
~2 mg/mL
technique(s)
flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
indirect ELISA: suitable
western blot: 0.5-1 μg/mL using recombinant Cre recombinase
isotype
IgG1
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
unmodified
General description
Monoclonal Anti-Cre (mouse IgG1 isotype) is derived from the 7-23 hybridoma produced by the fusion of mouse myeloma cells (X63-Ag8.653) and splenocytes from BALB/c mice immunized with Cre protein. Cre recombinase is a member of the integrase family of proteins that are site-specific recombinases.The protein is expressed in P1 bacteriophage.
Application
Anti-Cre antibody ,Mouse monoclonal has been used in:
- enzyme linked immunosorbent assay (ELISA)
- immunoblotting
- immunoprecipitation
- immunohistochemistry
- immunocytochemistry
- flow cytometry
- immunostaining
Biochem/physiol Actions
Cre recombinase enables DNA recombination between two recognition sites called loxP. Each one of these sites (34 bp long) contain two 13 bp palindromic flanking sequences and in between them is a core spacer sequence 8 bp long. The recombination event takes place within the spacer area of the two loxP sites. Cre/loxP system manipulates genes in mammalian systems by making deletions, gene replacements, insertions (knockin), conditional gene targeting (knockout), and point mutations. Monoclonal antibodies to the Cre protein are an important tool for the identification of Cre recombinase in different mammalian systems.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Storage and Stability
For continuous use, store at 2-8 °C for up to one month. For extended storage, the solution should be frozen in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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Directional resolution of synthetic holliday structures by the Cre recombinase
Lee L and Sadowski PD
Test, 276(33), 31092-31098 (2001)
Juan D Rodriguez et al.
Genetics, 207(1), 129-138 (2017-07-12)
Transvection is broadly defined as the ability of one locus to affect its homologous locus
Katsuhisa Matsuura et al.
The Journal of cell biology, 167(2), 351-363 (2004-10-20)
The concept of the plasticity or transdifferentiation of adult stem cells has been challenged by the phenomenon of cell fusion. In this work, we examined whether neonatal cardiomyocytes fuse with various somatic cells including endothelial cells, cardiac fibroblasts, bone marrow
Fernando H Biase et al.
iScience, 7, 16-29 (2018-09-30)
We developed the Rainbow-seq technology to trace cell division history and reveal single-cell transcriptomes. With distinct fluorescent protein genes as lineage markers, Rainbow-seq enables each single-cell RNA sequencing (RNA-seq) experiment to simultaneously decode the lineage marker genes and read single-cell
Francesco Trovato et al.
Nature communications, 11(1), 6194-6194 (2020-12-05)
Genetic mosaicism, a condition in which an organ includes cells with different genotypes, is frequently present in monogenic diseases of the central nervous system caused by the random inactivation of the X-chromosome, in the case of X-linked pathologies, or by
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