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Key Documents

C-44010

Sigma-Aldrich

Lymphocyte Separation Medium 1077

500 ml

Synonym(s):

Lymphocyte Medium, Separation Medium for Lymphocytes

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.71

form

aqueous solution

packaging

pkg of 500 ml

technique(s)

cell culture | mammalian: suitable

shipped in

ambient

storage temp.

room temp

General description

Lymphocyte Separation Medium 1077

Application

Lymphocyte Separation Medium 1077 is intended for the separation of the portion of vital mononuclear cells from whole blood, buffy coats, bone marrow and several other starting materials, e.g. crude cell preparations, by means of low density gradient centrifugation. Lymphocyte separation Medium has a density of 1.077 g/ml at 20°C. It is sterile, ready-to use and based on Ficoll 400 and sodium diatrizoate providing optimal physiological parameters and low cytotoxicity.

Quality

All lots of PromoCell Lymphocyte Separation Medium 1077 are subjected to comprehensive quality control tests. Each lot is routinely tested for function and physical parameters such as osmolality and pH level. Approved in-house lots are used as a reference. In addition, all lots have been tested for the absence of microbial contaminants (fungi, bacteria).

Storage and Stability

Lymphocyte Separation Medium 1077 is light-sensitive! Store the medium at room temperature in the dark immediately after arrival. Do not freeze. If stored properly, the product is stable until the expiry date stated on the label.

Subculture Routine

Click here for more information.

Pictograms

Corrosion
GHS05

Signal Word

Warning

Hazard Statements

H290,H315,H319

Hazard Classifications

Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2

Storage Class Code

8B - Non-combustible corrosive hazardous materials

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Articles

Primary Human Mononuclear Cells and Growth Media

Mononuclear cells isolated from umbilical cord blood and adult peripheral blood and maintenance medium optimized for short term cultivation. Protocols for handling, cell passaging, media, and product use.

Protocols

Promocell

Mononuclear cells isolated from umbilical cord blood and adult peripheral blood and maintenance medium optimized for short term cultivation. Protocols for handling, cell passaging, media, and product use.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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