C3777
Casein fluorescein isothiocyanate from bovine milk
Type II, essentially salt-free, lyophilized powder
Synonym(s):
FITC-casein
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About This Item
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type
Type II
Quality Level
form
essentially salt-free, lyophilized powder
extent of labeling
20-50 μg FITC per mg solid
solubility
water: 5 mg/mL, clear to hazy, yellow to orange
storage temp.
2-8°C
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General description
Casein is a major milk phospho-protein and constitutes about 80% of the total protein content in milk. Bovine milk is composed of four types of casein such as, αS1, αS2, β, and κ. Casein exists as micelles in milk.
Application
Casein fluorescein isothiocyanate from bovine milk has been used:
- as a substrate for semi-quantitative analysis of protease in Arabidopsis cells
- to determine the caseinolytic activity of secreted LasB (elastase), alkaline protease and protease IV
- for determining the SpeB (cysteine protease) proteolytic activity in Streptococcus pyogenes cells
highly sensitive protease substrate
Biochem/physiol Actions
Casein micelle system is involved in the prevention of pathological calcification of mammary glands.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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Protocols
Our General Protease Assay Procedures and Substrates overview.
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