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88190

Sigma-Aldrich

Tetrazolium Blue Chloride

≥90% (T), powder, suitable for microbiology

Synonym(s):

3,3′-(3,3′-Dimethoxy[1,1′-biphenyl]-4,4′-diyl)-bis(2,5-diphenyl-2H-tetrazolium) dichloride, 3,3′-Dianisole-4,4′-bis(3,5-diphenyltetrazolium chloride), BTC, Blue Tetrazolium chloride, TZ

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About This Item

Empirical Formula (Hill Notation):
C40H32Cl2N8O2
CAS Number:
1871-22-3
Molecular Weight:
727.64
Beilstein:
3894077
EC Number:
217-488-8
MDL number:
MFCD00040933
UNSPSC Code:
12352204
PubChem Substance ID:
329769640
NACRES:
NA.83

product name

Tetrazolium Blue Chloride, indicator for germination, suitable for microbiology, ≥90% (T)

grade

indicator for germination

Assay

≥90% (T)

form

powder

mp

255 °C (dec.) (lit.)

solubility

methanol: 0.5 g/10 mL ( hot)

application(s)

microbiology

SMILES string

[Cl-].[Cl-].COc1cc(ccc1-[n+]2nc(nn2-c3ccccc3)-c4ccccc4)-c5ccc(c(OC)c5)-[n+]6nc(nn6-c7ccccc7)-c8ccccc8

InChI

1S/C40H32N8O2.2ClH/c1-49-37-27-31(23-25-35(37)47-43-39(29-15-7-3-8-16-29)41-45(47)33-19-11-5-12-20-33)32-24-26-36(38(28-32)50-2)48-44-40(30-17-9-4-10-18-30)42-46(48)34-21-13-6-14-22-34;;/h3-28H,1-2H3;2*1H/q+2;;/p-2

InChI key

MUUHXGOJWVMBDY-UHFFFAOYSA-L

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Other Notes

Used in the assay of reducing sugars; Used in the preparation of DNA-cation complexes, immunoadsorbents for anti-DNA antibodies.

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H350

Precautionary Statements

P201 - P308 + P313

Hazard Classifications

Carc. 1B

Storage Class Code

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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V A DeBari et al.
Journal of immunological methods, 40(1), 89-94 (1981-01-01)
DNA can be precipitated as complexes with metal and organic cations. We have prepared these products using divalent metal cations (Cu2+, Mn2+, Ni2+, Zn2+, and Co2+) and ditetrazolium ions (blue tetrazolium and neotetrazolium). They are effective adsorbents, in vitro, for
J.P. Robin et al.
Sciences Des Aliments, 1, 233-233 (1981)
Christophe Pannecouque et al.
Nature protocols, 3(3), 427-434 (2008-03-08)
Since its first description 20 years ago, the tetrazolium-based colorimetric (MTT) assay using MT-4 cells for the detection of anti-HIV compounds has been widely used. This method, which remains popular, provides more information than more recently developed methods and, therefore
Y Mizutani et al.
The Journal of urology, 160(2), 561-570 (1998-07-29)
The resistance of bladder cancer to anticancer chemotherapeutic drugs is a major problem. Several immunotherapeutic approaches have been developed to treat drug-resistant tumor cells. The Fas antigen (Fas)-Fas ligand pathway is involved in cytotoxic T lymphocyte and natural killer cell-mediated
Chin-I Chang et al.
Sensors (Basel, Switzerland), 12(3), 2710-2728 (2012-06-28)
We coupled 16S rDNA PCR and DNA hybridization technology to construct a microarray for simultaneous detection and discrimination of eight fish pathogens (Aeromonas hydrophila, Edwardsiella tarda, Flavobacterium columnare, Lactococcus garvieae, Photobacterium damselae, Pseudomonas anguilliseptica, Streptococcus iniae and Vibrio anguillarum) commonly
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