Skip to Content
Merck
All Photos(1)

Key Documents

806552

SAFC

Phosphate Buffered Saline

pH 7.4, liquid, sterile-filtered, suitable for cell culture

Synonym(s):

Phosphate buffered saline, PBS

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

MDL number:
MFCD00131855
UNSPSC Code:
12352207
NACRES:
NA.75

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

Endotoxin, tested

pH

7.4

Looking for similar products? Visit Product Comparison Guide

General description

Phosphate-buffered saline (PBS) is a buffer solution used in biological research. It is a water-based salt solution containing sodium phosphate, sodium chloride and, in some formulations, it contains potassium chloride and potassium phosphate. The osmolality and ion concentrations of the solutions match those of the human body (isotonic) and are non-toxic to most cells.

Application

Phosphate-buffered saline has been used:
  • as a reagent in the isolation of human endometrial stem cell/stromal cells (hEnSCs) from the endometrium or menstrual blood
  • has also been used to wash cells during immunofluorescence staining
  • as a solvent for the glucose administered orally in the Glucose Tolerance Tests (GTTs)

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Human Endometrial Stem Cell Isolation from Endometrium and Menstrual Blood
Tavakol S, et al.
Molecular Neurobiology (2016)
L R Gabbert et al.
Journal of applied microbiology, 129(5), 1092-1101 (2020-05-08)
Develop an effective laboratory method to consistently recover viral loads from porous concrete coupons sufficient for disinfectant efficacy testing. Investigate the role of concrete matrix pH on the recovery of foot-and-mouth disease virus (FMDV) and African Swine Fever virus (ASFV)
Dieter Fink et al.
PloS one, 14(11), e0224576-e0224576 (2019-11-08)
Intra-bone marrow transplantation (IBMT) has been adapted for mouse models to improve the seeding efficiency of transplanted hematopoietic stem and progenitor cells. Commonly used injection volumes for IBMT into the tibia differ between 10 and 40 μL even though considerable
Qingyun Peng et al.
International journal of biological sciences, 16(15), 2974-2988 (2020-10-17)
Sepsis-induced myocardial dysfunction (SIMD) is a life-threatening complication caused by inflammation, but how it is initiated is still unclear. Several studies have shown that extracellular high mobility group box 1 (HMGB1), an important cytokine triggering inflammation, is overexpressed during the
Fang Xu et al.
Reproductive biology, 18(4), 344-354 (2018-11-14)
The sperm acrosome reaction (AR) is a physiological secretory course of membrane fusion and hydrolytic enzymes, as well as matrix protein release, enabling spermatozoa to penetrate the egg surroundings. An instable acrosomal status before a specific stimulus, insufficient acrosomal responsiveness
View All Related Papers

Protocols

Protocol Guide: WST-1 Assay for Cell Proliferation and Viability

WST-1 assay protocol for measuring cell viability, proliferation, activation and cytotoxicity. Instructions for WST-1 reagent preparation and examples of applications. Frequently asked questions and troubleshooting guide for WST-1 assay.

MTT Assay Protocol for Cell Viability and Proliferation
Protocol Guide: XTT Assay for Cell Viability and Proliferation

Protocol Guide: XTT Assay for Cell Viability and Proliferation

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service