41785

Dimidium bromide

BioReagent, suitable for fluorescence, ~95% (AT)

• Synonym(s): 3,8-Diamino-5-methyl-6-phenylphenanthridinium bromide, Trypadine, phenanthridinium 1553
• Empirical Formula (Hill Notation): C₂₀H₁₈BrN₃
• CAS Number: 518-67-2
• Molecular Weight: 380.28
• Beilstein: 3809779
• EC Number: 208-256-7
• MDL number: MFCD00011757
• UNSPSC Code: 12171500
• PubChem Substance ID: 57650090
• NACRES: NA.32

Properties

• product line: BioReagent
• Assay: ~95% (AT)
• form: solid
• mp: 243-248 °C (lit.)
• fluorescence: λ_ex 306 nm; λ_em 612 nm in 50 mM Tris pH 8.0 (DNA); λ_ex 480 (pH 7.6, low ionic strength)
• suitability: suitable for fluorescence
• SMILES string: [Br-].C[n+]1c(-c2ccccc2)c3cc(N)ccc3c4ccc(N)cc14
• InChI: 1S/C20H17N3.BrH/c1-23-19-12-15(22)8-10-17(19)16-9-7-14(21)11-18(16)20(23)13-5-3-2-4-6-13;/h2-12,22H,21H2,1H3;1H
• InChI key: MQOKYEROIFEEBH-UHFFFAOYSA-N

Description

General description

Dimidium Bromide is a trypanocidal drug that is known to affect nucleic acid synthesis in many organisms e.g. tissue culture cells.

Application

Dimidium Bromide is used as a binding agent to natural DNA based on base-pair specificity. It is used as a fluorescence agent for nucleic acid experiments.

Intercalating probe for nucleic acids.

Safety Information

• Pictograms: GHS07
• Signal Word: Warning
• Hazard Statements: H315,H319,H335
• Precautionary Statements: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
• Target Organs: Respiratory system
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves