09111802

MS-1 cell line

• Synonym(s): MS1 Cells
• UNSPSC Code: 12352200

Properties

• biological source: human skin
• Quality Level: 100
• form: liquid
• growth mode: Suspension
• karyotype: Not determined
• morphology: Loosely packed floating aggregates with irregular outline and no central necrosis
• products: Cell line is CM2B4/MCV T, AE1/AE2, CK20, EMA, CAM5.2, chromogranin & NSE. Cell line is negative for for CK7, synaptophysin, LCA TTF-1, vimentin and CD99,
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Human neuroendocrine skin carcinoma

Cell Line Description

MS-1 cells contain a Merkel cell polyomavirus integrated into the genome. See also MKL-1 (Sigma Catalogue number 09111801).

Application

Pathobiology of neuroendocrine skin carcinomas (NSCs) and function of cutaneous Merkel cells. The role of polyomavirus in the development of Merkel cell carcinomas (submitted for publication)

DNA Profile

STR-PCR Data:
Amelogenin: X
CSF1PO: 10
D13S317: 11,14
D16S539: 9
D5S818: 11
D7S820: 8,9
THO1: 7,9.3
TPOX: 8
vWA: 17

Culture Medium

RPMI 1640 + 2mM Glutamine + 20% Foetal Bovine Serum (FBS).

Subculture Routine

Seed at 3 x 10⁵ cells/ml in 5% CO₂; 37°C Saturation density is approximately 10⁶ cells/ml.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Depositor and originator: Dr Patrick Moore, University of Pittsburgh Cancer Institute, 5117 Centre Avenue Suite 1.8 Pittsburgh PA 15213

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Safety Information

• Storage Class Code: 6.2 - Infectious substances
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable