T9284

Triton^™ X-100

BioXtra

• Synonym(s): t-Octylphenoxypolyethoxyethanol, Polyethylene glycol tert-octylphenyl ether
• Linear Formula: t-Oct-C₆H₄-(OCH₂CH₂)_xOH, x= 9-10
• CAS Number: 9036-19-5
• Beilstein: 2315025
• MDL number: MFCD00128254
• UNSPSC Code: 12161900
• PubChem Substance ID: 329826903
• NACRES: NA.21

Properties

• biological source: synthetic
• Quality Level: 200
• description: non-ionic
• product line: BioXtra
• form: liquid
• mol wt: micellar avg mol wt 80,000; average mol wt 625
• aggregation number: 100-155
• technique(s): permeability assay: suitable
• impurities: ≤0.05% Phosphorus (P)
• ign. residue: ≤0.4%
• CMC: 0.2-0.9 mM (20-25°C)
• transition temp: cloud point 65 °C; pour point ~7 °C
• solubility: water: soluble
• density: 1.065 g/cm³ at 20 °C (68 °F)
• anion traces: chloride (Cl^-): ≤0.05%; sulfate (SO₄^2-): ≤0.05%
• cation traces: Al: ≤0.0005%; Ca: ≤0.0005%; Cu: ≤0.0005%; Fe: ≤0.0005%; K: ≤0.005%; Mg: ≤0.0005%; NH₄⁺: ≤0.05%; Na: ≤0.1%; Pb: ≤0.001%; Zn: ≤0.0005%
• HLB: 13.5
• SMILES string: CC(C)(C)CC(C)(C)c1ccc(OCCOCCOCCOCCOCCOCCOCCO)cc1
• InChI: 1S/C28H50O8/c1-27(2,3)24-28(4,5)25-6-8-26(9-7-25)36-23-22-35-21-20-34-19-18-33-17-16-32-15-14-31-13-12-30-11-10-29/h6-9,29H,10-24H2,1-5H3
• InChI key: HNLXNOZHXNSSPN-UHFFFAOYSA-N

Description

General description

Triton^™ X-100 is a common non-ionic surfactant and emulsifier which is often used in biochemical applications to solubilize proteins. It is considered a comparatively mild detergent, non-denaturing, and is reported in numerous references as a routinely added reagent. It is utilized for lysing cells to extract protein and cellular organelles. It can also permeabilize the living cell membrane for transfection.

Application

Triton^™ X-100 has been used:

• Along with Span^® 80 and Tween^® 80 in mineral oil for the preparation of oil-phase of microemulsions for PCR technique
• As a component of NX buffer which is used to pool and break down the microemulsions after PCR cycling
• In the blocking solution during immunocytochemistry procedure
• During deglycosylation procedure
• For membrane solubilization
• For cell permeabilization
• In decellularization of porcine heart valve tissue

Biochem/physiol Actions

Widely used non-ionic surfactant for recovery of membrane components under mild non-denaturing conditions.

Features and Benefits

• BioXtra Grade product for your Cell Biology and Biochemical research
• Non-ionic nondenaturing detergent
• Superior wetting agent and emulsifier
• Highly versatile surfactant

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

For additional information on our range of Biochemicals, please complete this form.

Legal Information

Span is a registered trademark of Croda International PLC

TWEEN is a registered trademark of Croda International PLC

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

Safety Information

• Pictograms: GHS05,GHS07,GHS09
• Signal Word: Danger
• Hazard Statements: H302 - H315 - H318 - H410
• Precautionary Statements: P264 - P273 - P280 - P301 + P312 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Irrit. 2
• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point(F): 483.8 °F
• Flash Point(C): 251 °C
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves