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R8881

Sigma-Aldrich

Rhodamine B isothiocyanate–Dextran

average mol wt ~10,000

Synonym(s):

RITC-Dextran

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About This Item

MDL number:
UNSPSC Code:
12171500
NACRES:
NA.47

form

powder

Quality Level

mol wt

average mol wt ~10,000

extent of labeling

0.002-0.01 mol RITC per mol glucose

color

light pink to red
very dark purple

solubility

water: 10 mg/mL, clear, pink

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-8°C

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General description

Rhodamine B is a thiol-reactive and photostable fluorescent dye. Rhodamine B isothiocyanate is an aminoxanthene that possesses carboxylic acid and isothiocyanate substituents on the pendant phenyl ring.

Application

Rhodamine B isothiocyanate (NE)-labeled dextran is often used as a fluorescent probe for studies of endocytotic activity and to detect the migrated of marked cells. It is also suitable for perfusion studies in animals. Rhodamine B isothiocyanate–Dextran has been used for in vivo zebrafish blood-brain barrier (BBB) dye diffusion assay.
Dextran labeled with rhodamine B isothiocyanate for possible use in perfusion studies in animals.

Biochem/physiol Actions

Rhodamine B stains the tissue in red-violet color and emits a bright red color when fluoresced. It is highly preferred for marking insects.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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