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Key Documents

A9918

Sigma-Aldrich

Agarose

Type II-A, Medium EEO

Synonym(s):

3,6-Anhydro-α-L-galacto-β-D-galactan, Agarose ME

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About This Item

CAS Number:
EC Number:
MDL number:
UNSPSC Code:
41105317
PubChem Substance ID:
NACRES:
NA.25

biological source

algae (Gelidium)

Quality Level

type

Type II-A

form

powder

impurities

≤7% water

ash

≤0.5%

turbidity

≤40 NP (1.5% gel)

EEO

0.16-0.19

mp

86.5-89.5  °C

transition temp

gel point 36 °C ±1.5 °C (1.5% gel)

gel strength

≥1000 g/cm2 (1% gel)

anion traces

sulfate (SO42-): ≤0.25%

InChI

1S/C24H38O19/c25-1-5-9(27)11(29)12(30)22(38-5)41-17-8-4-36-20(17)15(33)24(40-8)43-18-10(28)6(2-26)39-23(14(18)32)42-16-7-3-35-19(16)13(31)21(34)37-7/h5-34H,1-4H2/t5-,6-,7+,8+,9+,10+,11+,12-,13+,14-,15+,16-,17-,18+,19+,20+,21-,22+,23+,24+/m1/s1

InChI key

MJQHZNBUODTQTK-WKGBVCLCSA-N

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Application


  • Porous Agarose Layered Magnetic Graphene Oxide Nanocomposite for Virus RNA Monitoring in Wastewater.: This innovative study showcases the use of agarose in the creation of a nanocomposite material for environmental monitoring, specifically for tracking viral RNA in wastewater (He et al., 2024).

  • Detection method for reverse transcription recombinase-aided amplification of avian influenza virus subtypes H5, H7, and H9.: Demonstrates the application of agarose in the development of diagnostic tools for detecting and monitoring avian influenza, emphasizing its critical role in enhancing bioanalytical methods (Zhang et al., 2024).

  • Evaluating Nanoparticles Penetration by a New Microfluidic Hydrogel-Based Approach.: This research utilizes agarose-based hydrogels in a microfluidic system to study nanoparticle penetration, offering insights into cellular interactions and nanoparticle dynamics that are essential for pharmaceutical development and nanotechnology (Goodarzi et al., 2024).

  • Antibacterial efficacy of copper-based metal-organic frameworks against Escherichia coli and Lactobacillus.: Agarose has been used to perform agarose gel electrophoresis for the synthesis of copper-based metal-organic frameworks, enhancing the antimicrobial properties of these compounds. This application points to the expanding role of agarose in antimicrobial research, relevant for both clinical and agricultural settings (Elmehrath et al., 2024).

Analysis Note

The following is a list of properties associated with our agaroses:
Sulfate content - used as an indicator of purity, since sulfate is the major ionic group present.
Gel strength - the force that must be applied to a gel to cause it to fracture.
Gel point - the temperature at which an aqueous agarose solution forms a gel as it cools. Agarose solutions exhibit hysteresis in the liquid-to-gel transition - that is, their gel point is not the same as their melting temperature.
Electroendosmosis (EEO) - a movement of liquid through the gel. Anionic groups in an agarose gel are affixed to the matrix and cannot move, but dissociable counter cations can migrate toward the cathode in the matrix, giving rise to EEO. Since electrophoretic movement of biopolymers is usually toward the anode, EEO can disrupt separations because of internal convection.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Jennifer K Roebber et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 39(32), 6224-6232 (2019-06-07)
How taste buds detect NaCl remains poorly understood. Among other problems, applying taste-relevant concentrations of NaCl (50-500 mm) onto isolated taste buds or cells exposes them to unphysiological (hypo/hypertonic) conditions. To overcome these limitations, we used the anterior tongue of
Franziska Koch et al.
BMC oral health, 20(1), 148-148 (2020-05-21)
Periodontitis is a chronic inflammation of the tooth supporting structures that finally can lead to tooth loss. As chronic periodontitis is associated with systemic diseases multiple approaches have been followed to support regeneration of the destructed tissue. But very few
Ryosuke Matsumura et al.
Scientific reports, 8(1), 9905-9905 (2018-07-04)
The electrical impedance of cell membranes is important for excitable cells, such as neurons, because it strongly influences the amount of membrane potential change upon a flow of ionic current across the membrane. Here, we report on an investigation of
Julia Dahlmann et al.
Biomaterials, 34(10), 2463-2471 (2013-01-22)
In most pluripotent stem cell differentiation protocols the formation of embryoid bodies (EBs) is an important step. Here we describe a rapid, straightforward soft lithography approach for the preparation of hydrophilic silicon masters from different templates and the subsequent production
Markus Landthaler et al.
RNA (New York, N.Y.), 14(12), 2580-2596 (2008-11-04)
microRNAs (miRNAs) regulate the expression of mRNAs in animals and plants through miRNA-containing ribonucleoprotein particles (RNPs). At the core of these miRNA silencing effector complexes are the Argonaute (AGO) proteins that bind miRNAs and mediate target mRNA recognition. We generated

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