A8097

Acridine Orange hydrochloride solution

>95.0% purity (HPLC), solution

• Synonym(s): 3,6-Bis(dimethylamino)acridine hydrochloride
• Empirical Formula (Hill Notation): C₁₇H₁₉N₃ · HCl
• CAS Number: 65-61-2
• Molecular Weight: 301.81
• Beilstein: 3575587
• MDL number: MFCD00012660
• UNSPSC Code: 12171500
• PubChem Substance ID: 24891317
• NACRES: NA.47

Properties

• product name: Acridine Orange hydrochloride solution, ≥95.0% (HPLC), 10 mg/mL in H₂O
• Quality Level: 200
• Assay: ≥95.0% (HPLC)
• form: solution
• concentration: 10 mg/mL in H₂O
• color: orange to red
• εmax: 47-54 at 486-492 nm in water
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: room temp
• SMILES string: Cl[H].CN(C)c1ccc2cc3ccc(cc3nc2c1)N(C)C
• InChI: 1S/C17H19N3.ClH/c1-19(2)14-7-5-12-9-13-6-8-15(20(3)4)11-17(13)18-16(12)10-14;/h5-11H,1-4H3;1H
• InChI key: VSTHNGLPHBTRMB-UHFFFAOYSA-N

Description

Application

Acridine Orange hydrochloride solution has been used to study autophagic cell death. It has also been used for the staining of chromosomes.

Biochem/physiol Actions

Acridine Orange is a metachromatic dye which can stain DNA, RNA and acid glycosaminoglycans. At low concentration it intercalates into DNA and precipitates RNA. However, at high concentration it denatures and precipitates both RNA and DNA. Acridine Orange is also used to analyze autophagy. It goes into acidic organelles in a pH-dependent manner. At neutral pH, acridine orange gives a green fluorescence and in acidic conditions, it accumulates in the acidic organelle giving a bright red fluorescence.

Safety Information

• Pictograms: GHS08
• Signal Word: Warning
• Hazard Statements: H341
• Precautionary Statements: P201 - P202 - P280 - P308 + P313 - P405 - P501
• Hazard Classifications: Muta. 2
• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves