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63068

Sigma-Aldrich

Magnesium chloride hexahydrate

BioUltra, for molecular biology, ≥99.0% (KT)

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About This Item

Linear Formula:
MgCl2 · 6H2O
CAS Number:
7791-18-6
Molecular Weight:
203.30
EC Number:
232-094-6
MDL number:
MFCD00149781
UNSPSC Code:
12352302
PubChem Substance ID:
329759771
NACRES:
NA.26

grade

for molecular biology

Quality Level

200

product line

BioUltra

Assay

≥99.0% (KT)

form

solid

impurities

DNases, none detected
 RNases, none detected
 insoluble matter, passes filter test
 phosphatases, none detected
 proteases, none detected
≤0.0002% total nitrogen (N)

pH

5.0-7.5 (25 °C, 1 M in H2O)

solubility

H2O: 1 M at 20 °C, clear, colorless

anion traces

phosphate (PO43-): ≤5 mg/kg
sulfate (SO42-): ≤50 mg/kg

cation traces

Al: ≤5 mg/kg
As: ≤0.1 mg/kg
Ba: ≤5 mg/kg
Bi: ≤5 mg/kg
Ca: ≤50 mg/kg
Cd: ≤5 mg/kg
Co: ≤5 mg/kg
Cr: ≤5 mg/kg
Cu: ≤5 mg/kg
Fe: ≤5 mg/kg
K: ≤2000 mg/kg
Li: ≤5 mg/kg
Mn: ≤5 mg/kg
Mo: ≤5 mg/kg
Na: ≤3000 mg/kg
Ni: ≤5 mg/kg
Pb: ≤5 mg/kg
Sr: ≤5 mg/kg
Zn: ≤5 mg/kg

λ

1 M in H2O

UV absorption

λ: 260 nm Amax: 0.030
λ: 280 nm Amax: 0.025

SMILES string

O.O.O.O.O.O.Cl[Mg]Cl

InChI

1S/2ClH.Mg.6H2O/h2*1H;;6*1H2/q;;+2;;;;;;/p-2

InChI key

DHRRIBDTHFBPNG-UHFFFAOYSA-L

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Application

Magnesium chloride hexahydrate has been used as a component of:
  • alkaline tris buffer to prepare labeling solution and to wash zebrafish embryos
  • HEPES-Tyrode solution for imaging cardiomyocyte cells
  • respiration solution, adenosine diphosphate (ADP) stock solution and wash solution to assess the respiration of permeabilized cardiomyocytes from mice

Caution

may agglomerate

Other Notes

Activation of restriction endonucleases

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
BCCH1092
BCCG9371
SLCJ1472
BCCG2643
SLCH6033

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J E Brooks
Methods in enzymology, 152, 113-129 (1987-01-01)
It is clear that we have still not exhausted all the restriction endonuclease specificities to be found in nature. Recently discovered BsmI is the first endonuclease recognizing a nonpalindromic sequence that cleaves within the site. Certainly other endonucleases belonging to
Liang Yang et al.
International journal of pharmaceutics, 471(1-2), 258-263 (2014-05-20)
Telmisartan (TEL) belongs to BCS class II (low solubility/high permeability) and exhibits the pH-dependent drug release. Since 3-aminopropyl functionalized magnesium phyllosilicate (aminoclay) can intercalate or adsorb the negatively charged molecules via the electrostatic interaction, TEL-aminoclay complex was synthesized to improve
Günther Gratzl et al.
Colloids and surfaces. B, Biointerfaces, 126, 98-105 (2014-12-30)
The availability of polymeric antimicrobially active surfaces, which are mainly based on cationic surface effects, is limited. We have previously reported the discovery that, in addition to cationic surfaces, anionic surfaces based on poly(acrylic acid) (PAA) copolymers have a bactericidal
Monica Mazzarino et al.
Analytica chimica acta, 837, 70-82 (2014-07-09)
This article describes a liquid chromatographic/tandem mass spectrometric method, based on the use of precursor ion scan as the acquisition mode, specifically developed to detect indole-derived cannabinoids (phenylacetylindoles, naphthoylindoles and benzoylindoles) in biological fluids (saliva, urine and blood). The method
Ikuo Yamamiya et al.
Drug metabolism and disposition: the biological fate of chemicals, 42(9), 1485-1492 (2014-07-09)
Tegafur (FT), a prodrug of 5-fluorouracil, is a chiral molecule, a racemate of R- and S-isomers, and CYP2A6 plays an important role in the enantioselective metabolism of FT in human liver microsomes (R-FT > S-FT). This study examined the enantioselective
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