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Roche

BM Chemiluminescence ELISA Substrate (AP)

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About This Item

UNSPSC Code:
12352204

usage

sufficient for 1,000 tests (ELISA)
 sufficient for 600 tests (SEAP)

packaging

pkg of 150 mL

manufacturer/tradename

Roche

shipped in

wet ice

storage temp.

2-8°C

Specificity

The AP assay system is designed for measuring alkaline phosphatase of various sources (e.g. intestine, placenta, milk, bacteria).

Features and Benefits

  • Sensitive: Approximately 10fg intestinal alkaline phosphatase can be detected
  • High dynamic measuring range: Linear range of more than five orders of magnitude
  • Constant light emission: The assay produces a long-lasting light emission instead of a short peak kinetics

Components

1. Alkaline Phosphatase Substrate, CSPD
2. Enhancer, Emerald II
3. Assay Buffer

Principle

CSPD is dephosphorylated by alkaline phosphatase (AP). The resulting instable dioxetane anion decomposes and emits light at a maximum wavelength of 477nm. Chemiluminescence-enhancing reagents improve the quantum yield of the excited state by more than 500 fold.

Preparation Note

Working solution: Preparation of working solution

The substrate reagent is composed of AP-substrate (bottle 1), Enhancer (bottle 2) and Assay Buffer (bottle 3). Prepare substrate reagent freshly before use. The ratio of the components depends on the application. The substrate reagent is stable at least for one day at 2 to 8 °C.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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JunBae Jee et al.
Journal of clinical microbiology, 42(12), 5664-5672 (2004-12-08)
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Martijn J Wilmer et al.
Cell and tissue research, 339(2), 449-457 (2009-11-11)
Reabsorption of filtered solutes from the glomerular filtrate and excretion of waste products and xenobiotics are the main functions of the renal proximal tubular (PT) epithelium. A human PT cell line expressing a range of functional transporters would help to

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