11684302001
Roche
ABTS™ Solution
solution (ready-to-use), suitable for ELISA
Synonym(s):
ABTS™ Solution, ABTS
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About This Item
UNSPSC Code:
12352204
Recommended Products
form
solution (ready-to-use)
Quality Level
usage
sufficient for 1,500-3,000 assays (ELISA)
packaging
pkg of 3 × 100 mL
manufacturer/tradename
Roche
technique(s)
ELISA: suitable
storage temp.
2-8°C
General description
ABTS™ Solution is a ready to use substrate for peroxidase-driven indicator reactions. It contains 2,2-azino-di-[3-ethylbenzthiazoline sulfonate (6)] and H2O2 in glycin/citric acid buffer and is available in slightly green color.
Application
ABTS Solution is a chromogenic substrate for peroxidase in ELISA assays.
Biochem/physiol Actions
2,2′-azino-di-(3-ethylbenzthiazoline sulfonic acid) or ABTS™ acts a substrate for HRP (horseradish peroxidase) conjugate during enzyme-linked immunosorbent assay (ELISA). It is the most sensitive, and stable substrate when compared to three other substrates namely, 5-aminosalicylic acid (5AS), O-phenylenediamine (OPD), O-tolidine (OT). It also produces the best visual results, where it gives a bluish-green color. ELISA using ABTS™ is a highly sensitive, specific and reproducible technique This solution is an excellent substrate for enzyme immunoassays with horseradish peroxidase as a marker enzyme.
Physical form
Reaction product:
Color: green
Evaluation: photometric (405nm)
Color: green
Evaluation: photometric (405nm)
Analysis Note
Absorption: ABTS absorption spectrum reference must always be measured and is determined by a spectrum. The Reference wavelength of 490 nm or higher should be selected due to no measureable absorption. The Reference absorption is usually automatically subtracted by the Plate Reader (background).
Other Notes
For life science research only. Not for use in diagnostic procedures.
Legal Information
ABTS is a trademark of Roche
WGK
nwg
Flash Point(F)
does not flash
Flash Point(C)
does not flash
Certificates of Analysis (COA)
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A micro-technique of enzyme-linked immunosorbent assay (ELISA) using ABTS, 2,2'-azino-di-(3-ethylbenzthiazoline sulfonic acid), as a substrate for horseradish peroxidase (HRP) conjugate was studied. In a comparative study among 4 substrates, namely; 5-aminosalicylic acid (5AS), O-phenylenediamine (OPD), O-tolidine (OT) and ABTS, for
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