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Roche

Mycoplasma PCR ELISA

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About This Item

UNSPSC Code:
12352200

usage

sufficient for 96 reactions

manufacturer/tradename

Roche

shipped in

dry ice

storage temp.

−20°C

Specificity

The Mycoplasma PCR ELISA is known to detect all of the following mycoplasma, acholeplasma, and ureaplasma species: M. orale, M. arginini, M. fermentans, M. hyorhinis, M. salivarium, M. gallisepticum, M. hominis, M. bovis, M. californicum, M. bovoculi, M. Pg50 bovine group, M. bovigenitalium, M. hyopneumoniae, A. laidlawii, U. urealyticum. DNA from other bacteria (except for Clostridium sp.), yeast, and eukaryotic cells are not detected.

Application

For research use only. Not for use in diagnostic procedures.
Mycoplasma PCR ELISA kit has been used for the detection of contamination by mycoplasma/acholeplasma/ureaplasma in cell cultures or other sample materials.
Mycoplasma PCR ELISA has been used to test mycoplasma contamination in cell lines.

Features and Benefits

Assay time: approximately 6 hours
Sample material: Cell culture supernatants, cell suspensions, or other samples
Sensitivity: At least 1 x 103 colony-forming units (cfu) of mycoplasma per milliliter of culture medium
  • High sensitivity: Detects 1 - 10fg of DNA, corresponding to approximately 1 - 20 gene copies per reaction (at least 103cfu/ml)
  • Broad detection range: All mycoplasma, acholeplasma, and ureaplasma species tested thus far were detected
  • Simplicity: Detects all mycoplasma using the same protocol, as opposed to the culture method that requires different culture conditions for different mycoplasma species
  • Fast results: The entire procedure can be performed in one day
  • Easy handling of large numbers of samples: The ready-to-use mix and ELISA detection procedure allow fast and semi-automatic sample processing

Packaging

1 kit containing 14 components.

Sequence

Chain Length Positive control: 520 bp.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Kit Components Only

Product No.
Description
  • Lysis Reagent
  • Neutralization Reagent
  • PCR Mix ready-to-use
  • Denaturation Reagent
  • Hybridization Buffer
  • Biotinylated Capture Probe
  • Washing Buffer 10x concentrated
  • Anti-Digoxigenin-POD antibody
  • Conjugate Dilution Buffer
  • TMB Substrate Solution
  • Stop Reagent
  • Positive Control DNA
  • Streptavidin-coated Microplate
  • Adhesive Plate Cover Foils
See All (14)

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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