MABT1547
Anti-CFAP206 Antibody, clone ORF2-2A7
Synonym(s):
Cilia- and flagella-associated protein 206
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
Recommended Products
biological source
rat
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
ORF2-2A7, monoclonal
mol wt
calculated mol wt 70.95 kDa
observed mol wt ~70 kDa
purified by
using protein G
species reactivity
mouse
packaging
antibody small pack of 100 μL
technique(s)
western blot: suitable
isotype
IgG2aκ
epitope sequence
C-terminal
Protein ID accession no.
UniProt accession no.
storage temp.
2-8°C
Gene Information
mouse ... Cfap206(69329)
Specificity
Clone ORF2-2A7 is a rat monoclonal antibody that detects Cilia- and flagella-associated protein 206 (CFAP206). It targets an epitope within 13 amino acids from the C-terminal region.
Immunogen
A linear peptide corresponding to 13 amino acids from the C-terminal region of mouse Cilia- and flagella-associated protein 206 (CFAP206).
Application
Quality Control Testing
Evaluated by Western Blotting in Mouse testis tissue extract.
Western Blotting Analysis: A 1:500 dilution of this antibody detected CFAP206 in Mouse testis tissue extract.
Tested Applications
Western Blotting Analysis: A representative lot detected CFAP206 in Western Blotting application. (Beckers, A., et al. (2020). Development. 147(21):dev188052).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Evaluated by Western Blotting in Mouse testis tissue extract.
Western Blotting Analysis: A 1:500 dilution of this antibody detected CFAP206 in Mouse testis tissue extract.
Tested Applications
Western Blotting Analysis: A representative lot detected CFAP206 in Western Blotting application. (Beckers, A., et al. (2020). Development. 147(21):dev188052).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Target description
Cilia- and flagella-associated protein 206 (UniProt: Q6PE87; also known as CFAP206) is encoded by the Cfap206 gene (Gene ID: 69329) in murine species. CFAP206 is an evolutionarily conserved protein that lacks known motifs or domains, except for a unique 280 amino acid domain with conserved motifs GFC and GIL. It localizes to the basal body/ centrosome and to cilia, and its expression correlates with and depends on Foxj1 expression. In vertebrates it plays a role during motile cilia assembly, and its deficiency can lead to abnormal ciliary beating and decreased sperm motility. It is reported to be a potential component of the nexin-dynein regulatory complex and interacts with radial spoke protein 3. Two isoforms of CFAP206 have been described that are produced by alternative spicing. Isoform 1 is expressed in the sperm, oviduct, lung, nasal cavity, brain ependyma, and choroid plexus. This isoform is shown to be essential for sperm motility and is involved in the regulation of the beating frequency of motile cilia on the epithelial cells of the respiratory tract and it is required for the establishment of radial spokes in sperm flagella. Isoform 2 that is expressed in the sperm, oviduct, lung, nasal cavity, brain ependyma, and choroid plexus is also required for sperm motility and is involved in the regulation of the beating frequency of motile cilia on the epithelial cells of the respiratory tract. Clone ORF2-2A7 specifically detects isoform 1 and detects a sequence within 13 amino acids from the C-terminal region, which is missing in isoform 2. (Ref.: Beckers, A., et al. (2020). Development. 147(21); dev188052).
Physical form
Purified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Reconstitution
0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Storage and Stability
Recommended storage: +2°C to +8°C.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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