MABF1479

Anti-CD120b/TNFR2 Antibody, clone TR75-89

• Synonym(s): TNF-R2, TNF-RII, TNFR-II, Tumor necrosis factor receptor 2, Tumor necrosis factor receptor superfamily member 1B, Tumor necrosis factor receptor type II, p75, p80 TNF-alpha receptor
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: hamster
• Quality Level: 200
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: TR75-89, monoclonal
• mol wt: calculated mol wt 50.32 kDa; observed mol wt ~N/A kDa
• purified by: using protein G
• species reactivity: mouse
• packaging: antibody small pack of 100
• technique(s): flow cytometry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• isotype: IgG
• epitope sequence: Extracellular domain
• Protein ID accession no.: NP_035740
• UniProt accession no.: P25119
• storage temp.: 2-8°C
• Gene Information: mouse ... Tnfrsf1b(21938)

Description

Specificity

Clone TR75-89 is an Armenian hamster monoclonal antibody that detects Tumor necrosis factor receptor 2/CD120b. It targets an epitope within the extracellular domain.

Immunogen

Purified extracellular domain of mouse Tumor necrosis factor receptor 2 (CD120b).

Application

Quality Control Testing

Evaluated by Flow Cytometry in L929 cells.

Flow Cytometry Analysis: 1.0 µg of this antibody detected CD120b/TNFR2 in one million L929 cells.

Tested Applications

Western Blotting Analysis: A representative lot detected CD120b/TNFR2 in Western Blotting applications (Sheehan, K.C., et al. (1995). J Exp Med. 181(2):607-17).

Immunoprecipitation Analysis: A representative lot detected CD120b/TNFR2 in Immunoprecipitation applications (Pinckard, J.K., et al. (1997). J Immunol. 158(8):3869-73).

Flow Cytometry Analysis: A representative lot detected CD120b/TNFR2 in Flow Cytometry applications (Kim, E.Y., et al. (2004). J Immunol. 173(7):4500-9).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Target description

Tumor necrosis factor receptor superfamily member 1B (UniProt: P25119; also known as Tumor necrosis factor receptor 2, TNF-R2, Tumor necrosis factor receptor type II, TNF-RII, TNFR-II, p75, p80 TNF-alpha receptor, CD120b) is encoded by the Tnfrsf1b (also known as Tnfr-2, Tnfr2) gene (Gene ID: 21938) in murine species. TNFR2 is a single-pass type I membrane glycoprotein that is synthesized with a signal peptide (aa 1-22), which is subsequently cleaved off to produce the mature form that contains an extracellular domain (aa 23-258), a transmembrane domain (aa 259-288), and a cytoplasmic domain (aa 289-474). It serves as a high-affinity receptor for TNF- . It displays about five-fold reduced affinity for lymphotoxin- . It acts as a co-stimulator for IL-2 Induction and T cell survival and enhances the responses of lymphocytes to TCR-mediated signaling. It plays a critical role during TCR/CD28-mediated stimulation toward IL-2 induction, clonal expansion, and survival. TNFR2 deficient T cells possess a profound defect in IL-2 production in response to TCR/CD28-mediated stimulation. It is also reported to induce proliferative responses in certain cells of hematopoietic origin and enhances expression of certain adhesion molecules such as ICAM-1. (Ref.: Chen, X., et al. (2010). J. Immunol. 185(1); 174-182; Kim, EY., and Teh, HS. (2004). J. Immunol. 173(7); 4500-4509; Sheehan, KCF., et al. (1995). J. Exp. Med. 181(2); 607-617).

Physical form

Purified Armenian hamster monoclonal antibody IgG in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Reconstitution

1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommended storage: +2°C to +8°C.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable