MABE893
Anti-LSm1 Antibody, clone 5F3
clone 5F3, from rat
Synonym(s):
U6 snRNA-associated Sm-like protein LSm1
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rat
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
5F3, monoclonal
species reactivity
mouse
technique(s)
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG1
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
mouse ... Lsm1(67207)
General description
The protein named U6 snRNA-associated Sm-like protein LSm1 or Cancer associated Sm-like, or Small nuclear ribonuclear CaSm and encoded by the human gene LSM1/CASM is a critical binding protein that mediates replication dependent histone mRNA degradation during cell division. Lsm1 is part of the Lsm family of proteins. Lsm proteins are a ubiquitous family of proteins characterized by the Sm-domain. They exist as hexa- or heptameric RNA-binding complexes and carry out RNA-related functions. Lsm1 interacts directly with SLBP a histone stem-loop binding protein critical for histone mRNA processing and expression. Histone mRNAs do not contain introns or polyadenylation signals and are processed by direct endonucleolytic cleavage. Lsm1 binds directly to the 3’-terminal U-tract of U6 snRNA . Lsm1 is localized to the nucleus and is up regulated in many cancers including pancreatic cancer and many tumor cell lines.
Immunogen
Recombinant protein corresponding to mouse LSm1.
Application
Anti-LSm1 Antibody, clone 5F3 is an antibody against LSm1 for use in western blotting & IP.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
RNA Metabolism & Binding Proteins
RNA Metabolism & Binding Proteins
Western Blotting Analysis: A representative lot from an independent laboratory detected LSm1 in MEF cell lysate (Hoefig, K. P., et al. (2012). Nat Struct Mol Biol. 20(1):73-81.).
Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated LSm1 from MEF cell lysate (Hoefig, K. P., et al. (2012). Nat Struct Mol Biol. 20(1):73-81.).
Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated LSm1 from MEF cell lysate (Hoefig, K. P., et al. (2012). Nat Struct Mol Biol. 20(1):73-81.).
Quality
Evaluated by Western Blotting in L-929 cell lysate.
Western Blotting Analysis: 1 µg/mL of this antibody detected LSm1 in 10 µg of L-929 cell lysate.
Western Blotting Analysis: 1 µg/mL of this antibody detected LSm1 in 10 µg of L-929 cell lysate.
Target description
~15 kDa observed
Physical form
Format: Purified
Protein G Purified
Purified rat monoclonal IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Kai P Hoefig et al.
Nature structural & molecular biology, 20(1), 73-81 (2012-12-04)
The exoRNase Eri1 inhibits RNA interference and trims the 5.8S rRNA 3' end. It also binds to the stem-loop of histone mRNAs, but the functional importance of this interaction remains elusive. Histone mRNAs are normally degraded at the end of
Poonam Dhillon et al.
Journal of virology, 92(24) (2018-09-28)
Rotavirus replicates in unique virus-induced cytoplasmic inclusion bodies called viroplasms (VMs), the composition and structure of which have yet to be understood. Based on the analysis of a few proteins, earlier studies reported that rotavirus infection inhibits stress granule (SG)
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