MABE636
Anti-monomethyl Histone H3 (Lys4) Antibody, clone RM140
clone RM140, from rabbit
Synonym(s):
Histone H3.3, Histone H3
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
antibody product type
primary antibodies
clone
RM140, monoclonal
species reactivity
human
technique(s)
multiplexing: suitable
western blot: suitable
isotype
IgG
UniProt accession no.
shipped in
wet ice
target post-translational modification
monomethylation (Lys4)
Gene Information
human ... H3F3B(3021)
General description
Histone H3, also known as Histone H3.1t (H3/t), H3t, H3/g, and encoded by the gene name HIST3H3/ H3FT, is a core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Therefore, histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. High levels of phosphorylation of Histone H3 are associated with mitosis.
Specificity
This antibody reacts to Histone H3 monomethylated at Lysine 4 (K4me1). No cross reactivity with dimethylated Lysine 4 (K4me2) or trimethylated Lysine 4 (K4me3), or other methylations in Histone H3.
Immunogen
A linear peptide corresponding to human Histone H3 monomethylated at Lys4.
Application
Multiplex Analysis (Mplex): 0.1-0.5 μg/mL from a representative lot was used in Multiplex.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Histone Modifying Proteins
Histone Modifying Proteins
This Anti-monomethyl Histone H3 (Lys4) Antibody, clone RM140 is validated for use in Western Blotting, Multiplexing for the detection of monomethyl Histone H3.
Quality
Evaluated by Western Blotting in Recombinant HeLa protein and HeLa acid extract.
Western Blotting Analysis (WB): 0.5 μg/mL of this antibody detected monomethyl Histone H3 (Lys4) in HeLa acid extract.
Western Blotting Analysis (WB): 0.5 μg/mL of this antibody detected monomethyl Histone H3 (Lys4) in HeLa acid extract.
Target description
~17 kDa observed. Uncharacterized bands may be observed in some lysate(s).
Physical form
Format: Purified
Protein A purified
Rabbit monoclonal in PBS with 1% BSA and 0.09% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10-13 - German Storage Class 10 to 13
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Jimsheena V Karakkat et al.
Nucleic acids research, 47(19), 10086-10103 (2019-09-19)
The metabolic sensor Per-Arnt-Sim (Pas) domain-containing serine/threonine kinase (PASK) is expressed predominantly in the cytoplasm of different cell types, although a small percentage is also expressed in the nucleus. Herein, we show that the nuclear PASK associates with the mammalian
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