MAB4051
Anti-Angiotensin Converting Enzyme Antibody, clone 9B9
clone 9B9, Chemicon®, from mouse
Synonym(s):
ACE, CD143, DCP1, Peptidyl-dipeptidase A, Peptidase P
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
9B9, monoclonal
species reactivity
hamster, feline, human, monkey, rat
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG1
suitability
not suitable for immunohistochemistry (Paraffin)
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... AGT(183)
General description
The previously assigned protein identifier P22966 has been merged into P12821. Full details can be found on the UniProt database.
Specificity
Angiotensin-converting enzyme [ACE] (N-terminal), no reactivity to testicular/germinal form of human ACE (90kDa isoform)
Immunogen
Angiotensin-converting enzyme from human lung
Application
Anti-Angiotensin Converting Enzyme Antibody, clone 9B9 detects level of Angiotensin Converting Enzyme & has been published & validated for use in ELISA, IP, WB, IC, IH.
Immunohistochemistry (Danilov et al., 1987; Falkenhahn et al., 1995; Morrell et al., 1995) : 1:1,000 on fresh frozen or 4% paraformaldehyde fixed tissues. Does not work on paraffin embedded tissue.
Immunocytochemistry: 1:100. Reacts with endothelial cell, macrophages (activated) and THP1 cells (monocyte line).
Immunoblotting: 1:100: ~170-180kDa isoform, reducing conditions.
Immunoprecipitation: 1:1,000-1:10,000
ELISA : 1:1,000
Visualization of lung vessels (Danilov et al., 1989; Klibanov et al., 1988; Hiemisch et al., 1993)
Antibody mediated drug delivery into the lung (Danilov et al., 1991, 1994; Muzykantov et al., 1994, 1996)
Estimation of lung vessel injury (Muzykantov et al., 1991; Atochina et al., 1992)
Structure-function of ACE (Danilov et al., 1994)
Optimal working dilutions must be determined by the end user.
Immunocytochemistry: 1:100. Reacts with endothelial cell, macrophages (activated) and THP1 cells (monocyte line).
Immunoblotting: 1:100: ~170-180kDa isoform, reducing conditions.
Immunoprecipitation: 1:1,000-1:10,000
ELISA : 1:1,000
Visualization of lung vessels (Danilov et al., 1989; Klibanov et al., 1988; Hiemisch et al., 1993)
Antibody mediated drug delivery into the lung (Danilov et al., 1991, 1994; Muzykantov et al., 1994, 1996)
Estimation of lung vessel injury (Muzykantov et al., 1991; Atochina et al., 1992)
Structure-function of ACE (Danilov et al., 1994)
Optimal working dilutions must be determined by the end user.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Hormones & Receptors
Hormones & Receptors
Physical form
Format: Purified
Purified immunoglobulin. Liquid in PBS, pH 7.4, 150 mM NaCl containing 0.05% sodium azide.
Storage and Stability
Maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Structure-function analysis of angiotensin I-converting enzyme using monoclonal antibodies. Selective inhibition of the amino-terminal active site.
Danilov, S, et al.
The Journal of Biological Chemistry, 269, 26806-26814 (1994)
Lung is the target organ for a monoclonal antibody to angiotensin-converting enzyme.
Danilov, S M, et al.
Laboratory Investigation; a Journal of Technical Methods and Pathology, 64, 118-124 (1991)
V V Petrov et al.
Journal of the renin-angiotensin-aldosterone system : JRAAS, 1(4), 342-352 (2002-04-23)
Appearance of angiotensin-converting enzyme (ACE) in fibrotic tissue can be the result of the action either of one particular growth factor or of cross-talk between multiple factors. Transforming growth factor-beta1 (TGF-beta(1)) is an effective inducor of the differentiation of cultured
Immunohistochemical study of angiotensin-converting enzyme in human tissues using monoclonal antibodies.
Danilov, S M, et al.
Histochemistry, 87, 487-490 (1987)
N W Morrell et al.
The Journal of clinical investigation, 96(4), 1823-1833 (1995-10-01)
Previous studies suggest that while lung angiotensin converting enzyme (ACE) activity is reduced during chronic hypoxia, inhibitors of ACE attenuate hypoxic pulmonary hypertension. In an attempt to explain this paradox we investigated the possibility that whole lung ACE activity may
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