AP184A

Donkey Anti-Sheep IgG Antibody, Alkaline Phosphatase conjugate, Species Adsorbed

0.6 mg/mL, Chemicon^®

• UNSPSC Code: 12352203
• eCl@ss: 32160702

Properties

• biological source: donkey
• Quality Level: 100
• conjugate: alkaline phosphatase conjugate
• antibody form: affinity purified immunoglobulin
• antibody product type: secondary antibodies
• clone: polyclonal
• species reactivity: sheep
• manufacturer/tradename: Chemicon^®
• concentration: 0.6 mg/mL
• technique(s): ELISA: suitable; western blot: suitable
• shipped in: wet ice
• target post-translational modification: unmodified

Description

General description

Alkaline Phosphatase-conjugated Affinity Purified Donkey anti Goat IgG (H+L)

Specificity

Based in immunoelectrophresis, this antibody reacts with the heavy and light chains on sheep IgG and with light chains common to most sheep immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. This antibody has been tested by Elisa and/ or solid phase absorbed to ensure minimal cross reactivity with chicken, guinea pig, Syrian Hamster, horse, human, mouse, rabbit, and rat serum proteins. Antibodies may cross react with immunoglobulins from other species.

Application

1:5,000 - 1:50,000 for Western Blot and Elisa

Optimal working dilutions must be determined by end user.

Donkey anti-Sheep IgG Antibody, Alkaline Phosphatase conjugate, Species Adsorbed detects level of Donkey Sheep IgG & has been published & validated for use in ELISA & WB.

Research Category
Secondary & Control Antibodies

Research Sub Category
Secondary Antibodies Adsorbed for Dual Labeling

Linkage

Replaces: AP305A

Physical form

ImmunoAffinity Purified

RECONSTITUTION:

Reconstitute with sterile distilled water to match the volume indicated on the vial label. Centrifuge product if it is not completely clear after standing for 1-2 hours at room temperature.

Storage and Stability

Maintain lyophilized product at 2-8°C for up to 12 months. After reconstitution the product is stable for several weeks at 2-8°C as an undiluted liquid. After dilution do not use for more than one day. For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of protein (and buffer salts) will decrease to one-half of the original after the addition of glycerol. Avoid repeated freeze/thaw cycles.

WARNING:

For research use only; not for use as a diagnostic.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Hazard Statements: H412
• Precautionary Statements: P273 - P501
• Hazard Classifications: Aquatic Chronic 3
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3