ABT241

Anti-acetyl Tubulin (Lys40) Antibody

from rabbit, purified by affinity chromatography

• Synonym(s): Tubulin alpha-1A chain, Alpha-tubulin 3, Tubulin B-alpha-1, Tubulin alpha-3 chain
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 100
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: human
• species reactivity (predicted by homology): rat (based on 100% sequence homology), bovine (based on 100% sequence homology), mouse (based on 100% sequence homology)
• technique(s): western blot: suitable
• NCBI accession no.: NP_006000
• UniProt accession no.: Q71U36
• shipped in: wet ice
• target post-translational modification: acetylation (Lys40)
• Gene Information: human ... TUBA1A(7846)

Description

General description

Tubulin is the major constituent of microtubules, and microtubules are major cytoskeletal elements. Tubulin proteins undergo a number of post-translational modifications both during individual synthesis and after complete tubule formation. Acetylation of alpha chains at Lys-40 stabilizes microtubules and affects the affinity and processivity of microtubule motors. This modification has a role in multiple cellular functions, ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking and signaling.

Immunogen

Epitope: Near N-Terminus

KLH-conjugated linear peptide corresponding to a region near the near N-Terminus of human Tubulin acetylated at Lys40.

Application

Anti-acetyl Tubulin (Lys40) Antibody is an antibody against acetyl Tubulin (Lys40) for use in western blotting.

Research Category
Cell Structure

Research Sub Category
Cytoskeleton

Quality

Evaluated by Western Blotting in untreated and Trichostatin A treated HeLa cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected acetyl Tubulin (Lys40) in 10 µg of Trichostatin A treated HeLa cell lysate, and not in untreated HeLa cell lysate.

Target description

~50 kDa observed

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable