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Key Documents

ABC459

Sigma-Aldrich

Anti-NOX4 Antibody

from rabbit

Synonym(s):

NADPH oxidase 4, Kidney oxidase-1, KOX-1, Kidney superoxide-producing NADPH oxidase, Renal NAD(P)H-oxidase

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

polyclonal

species reactivity

human, mouse

species reactivity (predicted by homology)

rat (based on 100% sequence homology)

technique(s)

immunocytochemistry: suitable
immunohistochemistry: suitable

NCBI accession no.

NP_001137308

UniProt accession no.

Q9NPH5

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... NOX4(50507)

General description

NOX4 (NADPH oxidase 4) is a phagocyte-type oxidase, similar to that responsible for the production of large amounts of reactive oxygen species (ROS) in neutrophil granulocytes with resultant antimicrobial activity and has been postulated to function in the kidney as an oxygen sensor that regulates the synthesis of erythropoietin in the renal cortex.

Immunogen

Linear peptide corresponding to the C-terminus of human NOX4.

Application

Anti-NOX4 Antibody is a highly specific rabbit polyclonal antibody, that targets NADPH Oxidase (phox) & has been tested in IHC & ICC.
Immunohistochemistry Analysis: A 1:500 and a 1:50 dilution from a representative lot detected NOX4 in human kidney and skeletal muscle tissues, respectively.

Immunohistochemistry Analysis:: A representative lot detected NOX4 in mouse liver tissue (I. Fabregat, IDIBELL, Barcelona, Spain).

Immunocytochemistry Analysis: A representative lot detected NOX4 in Hep3B cells (Esther Bertran, IDIBELL, Barcelona, Spain).

Immunohistochemistry Analysis: A representative lot detected NOX4 in mouse liver tissues expressing wildtype Mdr2/p19, or knockout Mdr2 ARF or knockout Stat3/Mdr2 (Sancho, P. et al. (2012). PLoS One. 7(9):e45285.).
Research Category
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional

Quality

Evaluated by Immunohistochemistry in human kidney tissue.

Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected NOX4 in human kidney tissue.

Target description

67 kDa calculated

Physical form

Format: Purified
Protein A purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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N A Porter et al.
Photochemistry and photobiology, 51(1), 37-43 (1990-01-01)
Human alpha-thrombin or bovine Factor Xa was acylated at the active site serine hydroxyl with alpha-methyl-2-hydroxy-4-diethylaminocinnamic acid. These modified serine proteinase enzymes showed no plasma coagulation biological activity in the absence of light. Photolysis of the acyl serine proteinase enzymes
Patricia Sancho et al.
PloS one, 7(9), e45285-e45285 (2012-10-11)
A role for the NADPH oxidases NOX1 and NOX2 in liver fibrosis has been proposed, but the implication of NOX4 is poorly understood yet. The aim of this work was to study the functional role of NOX4 in different cell
Joaquim Moreno-Càceres et al.
Cell death & disease, 8(10), e3098-e3098 (2017-10-13)
Hepatocellular carcinoma (HCC) is a heterogeneous tumour associated with poor prognostic outcome. Caveolin-1 (CAV1), a membrane protein involved in the formation of caveolae, is frequently overexpressed in HCC. Transforming growth factor-beta (TGF-β) is a pleiotropic cytokine having a dual role

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