238100

CRM1 Inhibitor III

The CRM1 Inhibitor III controls the biological activity of CRM1. This small molecule/inhibitor is primarily used for Cell Structure applications.

• Synonym(s): CRM1 Inhibitor III, (Z)-But-2-enedioic acid (4ʹ-bromophenyl)amide (3ʹʹ-chlorophenyl)amide, Chromosome Region Maintenance 1 ProteinInhibitor III, Exportin 1 Inhibitor III, XPO1 Inhibitor III
• Empirical Formula (Hill Notation): C₁₆H₁₂BrClN₂O₂
• CAS Number: 1178871-47-0
• Molecular Weight: 379.64
• MDL number: MFCD32671850
• UNSPSC Code: 12352200

Properties

• Quality Level: 100
• Assay: ≥95% (HPLC)
• form: powder
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; protect from light
• color: yellow
• solubility: DMSO: 100 mg/mL
• shipped in: wet ice
• storage temp.: 2-8°C
• SMILES string: O=C(NC1=CC=CC(Cl)=C1)/C=C\C(NC2=CC=C(Br)C=C2)=O

Description

General description

A cell-permeable butenediamide compound that acts as a potent, active site cysteine reactive irreversible inhibitor of CRM1/exportin 1. Shown to selectively repress agonist-mediated nuclear export of HDAC4/5, Rev and NFAT proteins in neonatal rat ventricular myocytes (NRVMs; EC₅₀ = 2.2 and 3.5 nM for GFP-HDAC5 and GFP-HIV Rev) and suppress hypertrophic growth of NRVMs (EC₅₀ = 52 nM for ANF expression inhibition) with negligible effect either on the phosphorylation of HDAC5 or on the kinase activities of PKD and CaMKII at 1 µM.

Packaging

Packaged under inert gas

Warning

Toxicity: Standard Handling (A)

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Other Notes

Monovich, L., et al. 2009. Biochim Biophys Acta.1789, 422.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable