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Supelco

Chromolith® RP-18 endcapped (monolithic) HPLC Columns

L × I.D. 100 mm x 3 mm HPLC Columns Validation Kit (3 Columns from 3 different batches)

Synonym(s):

Chromolith® RP-18 endcapped 100-3 HPLC columns Validation Kit (3 columns from 3 different batches)

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About This Item

UNSPSC Code:
41115700
NACRES:
SB.52

product name

Chromolith® RP-18 endcapped, L × I.D. 100 mm x 3 mm HPLC columns Validation Kit (3 columns from 3 different batches)

material

PEEK column

Quality Level

Agency

suitable for USP L1

product line

Chromolith®

feature

endcapped

packaging

pkg of 3 ea

extent of labeling

18% Carbon loading

parameter

200 bar pressure
45 °C max. temp.

technique(s)

HPLC: suitable
LC/MS: suitable
mass spectrometry (MS): suitable (High)

column L × I.D.

10 cm × 3 mm

matrix

monolithic silica

matrix active group

C18 bonding phase

pore size

13 nm mesoporosity
2 μm macroporosity

operating pH range

2-7.5

separation technique

reversed phase

storage temp.

no temp limit

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General description

Chromolith® RP-18 endcapped 100-3 HPLC columns Validation Kit consists of 3 columns from 3 different manufacturing batches.
Chromolith® columns are made of monolithic silica with a characteristic bimodal pore structure.

Chromolith® Performance RP-18 endcapped 100-3 columns have macropores with 2 μm diameter, giving a column efficiency exceeding 80,000 plates/meter. The mesopores are 13 nm (130 Å), the surface modification is octadecylsilane with full end-capping. Chromolith® Performance RP-18 endcapped 100-3 columns fall under the USP L1 Classification. The column efficiency is comparable to a 5 μm particle size fully porous particulate column. Chromolith® Performance RP-18 endcapped 100-3 columns are perfectly suitable for LC-MS applications.
The column dimension of 100 mm length and 3 mm I.D. is the best choice for fast separations of complex samples on any HPLC and UHPLC System.

The revolutionary, monolithic silica with its unique combination of macropores and mesopores enables:
—Rapid separations at very low column back-pressure
—High matrix tolerance, allowing time and cost savings with simplified workflows
—Cost-savings due to extended column lifetimes
—Connection of columns, flow gradients and the use of mobile phases with higher viscosity

The recommended Guard-columns are:
Chromolith® RP-18 endcapped 5-3 guard cartridges (3 pieces) [1.52005] with the Chromolith® Guard Cartridge Holder 5 mm x 3 mm [1.52004]
Holder and guard cartridges are also available as Guard cartridge set [1.52008]
The Chromolith® column coupler can be used to significantly increase the separation efficiency of Chromolith® columns by coupling several columns in series (up to 100,000 plates/per column). [1.51467]

Learn more:
Brochure: Race through your Separations on Any System. Chromolith® Monolithic Silica HPLC Columns

All Supelco HPLC Columns, including monolithic Chromolith® columns, are fully compatible to all HPLC and UHPLC Instruments.
Supelco HPLC Products: The Best Choice for Any LC Instrument

Legal Information

Chromolith is a registered trademark of Merck KGaA, Darmstadt, Germany

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Articles

Monolithic silica HPLC columns enable high-throughput analysis at low back pressure without loss of separation efficiency or peak capacity.

Chromolith® HPLC and UHPLC columns are made from monolithic silica with a bimodal pore structure using sol-gel technology.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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